ArticleMolecular reproduction and development2011
Caffeine alleviates the deterioration of Ca(2+) release mechanisms and fragmentation of in vitro-aged mouse eggs.
Article in Molecular reproduction and development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Antioxidant Supplementation with Caffeine During Rescue In Vitro Maturation Improves Fertilization and Embryo Development in Women of Advanced Maternal Age.Antioxidants (Basel, Switzerland) · 2026Article
- Iron metabolism disorder promotes postovulatory oocyte aging by inducing oxidative stress damage.Life medicine · 2025Article
- In Vivo-Matured Oocyte Resists Post-Ovulatory Aging through the Hub GenesAntioxidants (Basel, Switzerland) · 2024Article
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- Article
- In vivo and in vitro postovulatory aging: when time works against oocyte quality?Journal of assisted reproduction and genetics · 2022Review
- Meiotic Instability Generates a Pathological Condition in Mammalian Ovum.Stem cell reviews and reports · 2021Review
- Modulators of calcium signalling at fertilization.Open biology · 2020Review
- Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.Aging · 2020Article
- Postovulatory ageing modifies sperm-induced CaScientific reports · 2019Article
- Maintained MPF Level after Oocyte Vitrification Improves Embryonic Development after IVF, but not after Somatic Cell Nuclear Transfer.Molecules and cells · 2017Article
- Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes.Aging · 2017Article
- Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse.Oncotarget · 2017Article
- Biochemical alterations in the oocyte in support of early embryonic development.Cellular and molecular life sciences : CMLS · 2017Review
- Proteolytic fragmentation of inositol 1,4,5-trisphosphate receptors: a novel mechanism regulating channel activity?The Journal of physiology · 2016Review
- Increased level of reactive oxygen species persuades postovulatory aging-mediated spontaneous egg activation in rat eggs cultured in vitro.In vitro cellular & developmental biology. Animal · 2016Article
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- Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes.Human reproduction (Oxford, England) · 2016Article
- Effect of M-phase kinase phosphorylations on type 1 inositol 1,4,5-trisphosphate receptor-mediated Ca2+ responses in mouse eggs.Cell calcium · 2015Article
- Morphological, cellular and molecular changes during postovulatory egg aging in mammals.Journal of biomedical science · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The developmental competence of mammalian eggs is compromised by postovulatory aging. We and others have found that in these eggs, the intracellular calcium ([Ca(2+)](i)) responses required for egg activation and initiation of development are altered. Nevertheless, the mechanism(s) underlying this defective Ca(2+) release is not well known. Here, we investigated if the function of IP(3)R1, the major Ca(2+) release channel at fertilization, was undermined in in vitro-aged mouse eggs. We found that in aged eggs, IP(3)R1 displayed reduced function as many of the changes acquired during maturation that enhance IP(3)R1 Ca(2+) conductivity, such as phosphorylation, receptor reorganization and increased Ca(2+) store content ([Ca(2+)](ER)), were lost with increasing postovulatory time. IP(3)R1 fragmentation, possibly associated with the activation of caspase-3, was also observed in these eggs. Many of these changes were prevented when the postovulatory aging of eggs was carried out in the presence of caffeine, which minimized the decline in IP(3)R(1) function and maintained [Ca(2+)](ER) content. Caffeine also maintained mitochondrial membrane potential, as measured by JC-1 fluorescence. We therefore conclude that [Ca(2+)](i) responses in aged eggs are undermined by reduced IP(3)R1 sensitivity, decreased [Ca(2+)](ER) , and compromised mitochondrial function, and that addition of caffeine ameliorates most of these aging-associated changes. Understanding the molecular basis of the protective effects of caffeine will be useful in elucidating, and possibly reversing, the signaling pathway(s) compromised by in vitro culture of eggs.
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Registered trials
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