ArticleThe EMBO journal1990
Microtubule perturbation retards both the direct and the indirect apical pathway but does not affect sorting of plasma membrane proteins in intestinal epithelial cells (Caco-2).
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The trial behind it
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Who cites it
27 citing papers in PubMed, 109 citations in OpenAlex.
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Changes in αB-crystallin, tubulin, and MHC isoforms by hindlimb unloading show different expression patterns in various hindlimb muscles.Journal of exercise nutrition & biochemistry · 2014Article
- Cell cycle-dependent chromatin shuttling of HBO1-JADE1 histone acetyl transferase (HAT) complex.Cell cycle (Georgetown, Tex.) · 2014Article
- Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cytosolic, autocrine alpha-1 proteinase inhibitor (A1PI) inhibits caspase-1 and blocks IL-1β dependent cytokine release in monocytes.PloS one · 2012Article
- KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC.The Journal of cell biology · 2010Article
- Actomyosin contractility and microtubules drive apical constriction in Xenopus bottle cells.Developmental biology · 2007Article
- Transcriptional modulation of genes encoding structural characteristics of differentiating enterocytes during development of a polarized epithelium in vitro.Molecular biology of the cell · 2007Article
- Expression of a wild-type CFTR maintains the integrity of the biosynthetic/secretory pathway in human cystic fibrosis pancreatic duct cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2005Article
- (Glyco)sphingolipids are sorted in sub-apical compartments in HepG2 cells: a role for non-Golgi-related intracellular sites in the polarized distribution of (glyco)sphingolipids.The Journal of cell biology · 1998Article
- Regulation of cell polarity by microtubules in fission yeast.The Journal of cell biology · 1998Article
- Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells.Molecular biology of the cell · 1998Article
- Apiconuclear organization of microtubules does not specify protein delivery from the trans-Golgi network to different membrane domains in polarized epithelial cells.Molecular biology of the cell · 1998Article
- Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus.Journal of virology · 1996Article
- Membrane protein trafficking through the common apical endosome compartment of polarized Caco-2 cells.Molecular biology of the cell · 1995Article
- WIF-B cells: an in vitro model for studies of hepatocyte polarity.The Journal of cell biology · 1993Article
- Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant, F1-R, in epithelial MDCK cells.Journal of virology · 1993Article
- Regulation of cell surface polarity in renal epithelia.Pediatric nephrology (Berlin, Germany) · 1993Review
- Intracellular degradation and reduced cell-surface expression of sucrase-isomaltase in heat-shocked Caco-2 cells.The Biochemical journal · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous plasma membrane proteins are sorted from two sites in the human intestinal epithelial cell line Caco-2. Apical proteins are transported from the Golgi apparatus to the apical domain along a direct pathway and an indirect pathway via the basolateral membrane. In contrast, basolateral proteins never appear in the apical plasma membrane. Here we report on the effect of the microtubule-active drug nocodazole on the post-synthetic transport and sorting of plasma membrane proteins. Pulse-chase radiolabeling was combined with domain-specific cell surface assays to monitor the appearance of three apical and one basolateral protein in plasma membrane domains. Nocodazole was found to drastically retard both the direct transport of apical proteins from the Golgi apparatus and the indirect transport (transcytosis) from the basolateral membrane to the apical cell surface. In contrast, neither the transport rates of the basolateral membrane nor the sorting itself were significantly affected by the nocodazole treatment. We conclude that an intact microtubular network facilitates, but is not necessarily required for, the transport of apical membrane proteins along the two post-Golgi pathways to the brush border.
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