ArticleWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
The importance of a multifaceted approach to characterizing the microbial flora of chronic wounds.
Article in Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.
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Who cites it
72 citing papers in PubMed, 1 synthesis or guideline pooled it, 162 citations in OpenAlex.
- The Clinical Utility of Autofluorescence Imaging for Bacterial Detection in Wounds: A Systematic Review.International wound journal · 2025Pooled it
- Experimental Investigation of the Effects of 'Nigella sativa' and 'Vitis vinifera' on Intraoral Wound Healing.Oral health & preventive dentistry · 2026Article
- Impact of Bacterial Membrane Vesicles on Cellular Responses in Leishmania amazonensis-Infected Macrophages In Vitro.Scandinavian journal of immunology · 2026Article
- Use of TaqMan array card to investigate bacterial isolates in diabetic foot ulcers.Journal of medical microbiology · 2026Article
- Can commensals alter pathogen's antibiotic resistance during co-culture?Journal of medical microbiology · 2026Article
- Wounds and the Microbiota: The Healing Interplay Between Host and Microbial Communities.International journal of molecular sciences · 2025Review
- The Association of Systemic Inflammation, Wound Bioburden and Total Bacterial Counts With Healing Outcomes in Older Adults With Chronic Venous Leg Ulcers.International wound journal · 2025Article
- Staphylococcus caprae and Staphylococcus epidermidis define the skin microbiome among different grades of acne vulgaris.Archives of dermatological research · 2024Observational
- Host-Microbiome Crosstalk in Chronic Wound Healing.International journal of molecular sciences · 2024Review
- Article
- Review
- The Pathogen Isolates in Chronic Wound Infections in Poland.Polish journal of microbiology · 2023Article
- The Wound Microbiome.Cold Spring Harbor perspectives in biology · 2023Review
- Fluorescence In Situ Hybridization as Diagnostic Tool for Implant-associated Infections: A Pilot Study on Added Value.Plastic and reconstructive surgery. Global open · 2023Article
- Biofilm and wound healing: from bench to bedside.European journal of medical research · 2023Review
- Bacterial biofilms in the human body: prevalence and impacts on health and disease.Frontiers in cellular and infection microbiology · 2023Review
- Biofilm-Associated Infections in Chronic Wounds and Their Management.Advances in experimental medicine and biology · 2023Article
- Homocysteine and Inflammatory Cytokines in the Clinical Assessment of Infection in Venous Leg Ulcers.Antibiotics (Basel, Switzerland) · 2022Article
- The First Case of Preauricular Fistular Abscess Caused byAnnals of laboratory medicine · 2022Article
- The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes.PloS one · 2022Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 6 institutions in 1 country.
Funding
Abstract
Chronic wounds contain complex polymicrobial communities of sessile organisms that have been underappreciated because of limitations of standard culture techniques. The aim of this work was to combine recently developed next-generation investigative techniques to comprehensively describe the microbial characteristics of chronic wounds. Tissue samples were obtained from 15 patients with chronic wounds presenting to the Johns Hopkins Wound Center. Standard bacteriological cultures demonstrated an average of three common bacterial species in wound samples. By contrast, high-throughput pyrosequencing revealed increased bacterial diversity with an average of 17 genera in each wound. Data from microbial community profiling of chronic wounds were compared with published sequenced analyses of bacteria from normal skin. Increased proportions of anaerobes, Gram-negative rods and Gram-positive cocci were found in chronic wounds. In addition, chronic wounds had significantly lower populations of Propionibacterium compared with normal skin. Using epifluorescence microscopy, wound bacteria were visualized in highly organized thick confluent biofilms or as scattered individual bacterial cells. Fluorescent in situ hybridization allowed for the visualization of Staphylococcus aureus cells in a wound sample. Quorum-sensing molecules were measured by bioassay to evaluate signaling patterns among bacteria in the wounds. A range of autoinducer-2 activities was detected in the wound samples. Collectively, these data provide new insights into the identity, organization, and behavior of bacteria in chronic wounds. Such information may provide important clues to effective future strategies in wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.