ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
HCN channels expressed in the inner ear are necessary for normal balance function.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 75 citations in OpenAlex.
- Persistence of vestibular function in the absence of glutamatergic transmission from hair cells.Scientific reports · 2026Article
- Distribution of Voltage-Gated Sodium Channels and Scaffolding Proteins on Vestibular Calyx Ending Delineates the Axon Initial Segment.The Journal of comparative neurology · 2026Article
- The potassium channel subunit KeLife · 2024Article
- The potassium channel subunit KbioRxiv : the preprint server for biology · 2024Article
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- Electrophysiological properties of vestibular hair cells isolated from human crista.Frontiers in neurology · 2024Article
- Simultaneous recordings from vestibular Type I hair cells and their calyceal afferents in mice.Frontiers in neurology · 2024Article
- Modulating Hyperpolarization-Activated Cation Currents through Small Molecule Perturbations: Magnitude and Gating Control.Biomedicines · 2023Review
- Expression of hyperpolarization-activated current (Journal of neurophysiology · 2023Article
- Genome-wide association study reveals that the IBSP locus affects ear size in cattle.Heredity · 2023Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Nonquantal transmission at the vestibular hair cell-calyx synapse: KProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Differentiation Status of Hair Cells That Regenerate Naturally in the Vestibular Inner Ear of the Adult Mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- HCN channels in the mammalian cochlea: Expression pattern, subcellular location, and age-dependent changes.Journal of neuroscience research · 2021Article
- Similarities in the Biophysical Properties of Spiral-Ganglion and Vestibular-Ganglion Neurons in Neonatal Rats.Frontiers in neuroscience · 2021Article
- Efferent synaptic transmission at the vestibular type II hair cell synapse.Journal of neurophysiology · 2020Article
- Exocytosis in mouse vestibular Type II hair cells shows a high-order CaPhysiological reports · 2020Article
- Inhibitory Effective Perturbations of Cilobradine (DK-AH269), A Blocker of HCN Channels, on the Amplitude and Gating of Both Hyperpolarization-Activated Cation and Delayed-Rectifier Potassium Currents.International journal of molecular sciences · 2020Article
- Synaptic cleft microenvironment influences potassium permeation and synaptic transmission in hair cells surrounded by calyx afferents in the turtle.The Journal of physiology · 2020Article
- KNeuroscience · 2020Article
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4 authors at 3 institutions in 1 country.
Funding
Abstract
HCN1-4 subunits form Na+/K+-permeable ion channels that are activated by hyperpolarization and carry the current known as I(h). I(h) has been characterized in vestibular hair cells of the inner ear, but its molecular correlates and functional contributions have not been elucidated. We examined Hcn mRNA expression and immunolocalization of HCN protein in the mouse utricle, a mechanosensitive organ that contributes to the sense of balance. We found that HCN1 is the most highly expressed subunit, localized to the basolateral membranes of type I and type II hair cells. We characterized I(h) using the whole-cell, voltage-clamp technique and found the current expressed in 84% of the cells with a mean maximum conductance of 4.4 nS. I(h) was inhibited by ZD7288, cilobradine, and by adenoviral expression of a dominant-negative form of HCN2. To determine which HCN subunits carried I(h), we examined hair cells from mice deficient in Hcn1, 2, or both. I(h) was completely abolished in hair cells of Hcn1⁻/⁻ mice and Hcn1/2⁻/⁻ mice but was similar to wild-type in Hcn2⁻/⁻ mice. To examine the functional contributions of I(h), we recorded hair cell membrane responses to small hyperpolarizing current steps and found that activation of I(h) evoked a 5-10 mV sag depolarization and a subsequent 15-20 mV rebound upon termination. The sag and rebound were nearly abolished in Hcn1-deficient hair cells. We also found that Hcn1-deficient mice had deficits in vestibular-evoked potentials and balance assays. We conclude that HCN1 contributes to vestibular hair cell function and the sense of balance.
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