ArticleJournal of experimental botany2012
A male sterility-associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 141 citations in OpenAlex.
- Mitochondrial gene orf224 triggers retrograde signaling and reprogramming of anther cell fate in Brassica napus Polima CMS.The New phytologist · 2026Article
- 3-Hydroxypropanoic acid contributing to pollen abortion in ogura cytoplasmic male sterility of Brassica napus.BMC plant biology · 2026Article
- Genetic Resources of Cereal and Oilseed Crops for Heterotic Hybrid Breeding.Plants (Basel, Switzerland) · 2025Review
- Article
- Review
- Genome-Wide Identification of the SPP/SPPL Gene Family andInternational journal of molecular sciences · 2024Article
- Commercial suitability and characterization of newly developed Erucastrum canariense (Can) sterile cytoplasm based cytoplasmic male sterile (CMS) lines in Indian cauliflower.Scientific reports · 2024Article
- A breeding method for Ogura CMS restorer line independent of restorer source inFrontiers in genetics · 2024Article
- Mitochondrial genome comparison and phylogenetic analysis of Dendrobium (Orchidaceae) based on whole mitogenomes.BMC plant biology · 2023Article
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Cotton heterosis and hybrid cultivar development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Comparison of Mitochondrial Genomes between a Cytoplasmic Male-Sterile Line and Its Restorer Line for Identifying Candidate CMS Genes inInternational journal of molecular sciences · 2022Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (International journal of molecular sciences · 2022Article
- Comparative Cytological and Transcriptome Analyses of Anther Development inInternational journal of molecular sciences · 2022Article
- Article
- Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (International journal of molecular sciences · 2021Article
- A mitochondria-localized pentatricopeptide repeat protein is required to restore hau cytoplasmic male sterility in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility.BMC plant biology · 2021Article
- Orf165 is associated with cytoplasmic male sterility in pepper.Genetics and molecular biology · 2021Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect is often associated with a mitochondrial mutant. Approximately 10 chimeric genes have been identified as being associated with corresponding CMS systems in the family Brassicaceae, but there is little direct evidence that these genes cause male sterility. In this study, a novel chimeric gene (named orf288) was found to be located downstream of the atp6 gene and co-transcribed with this gene in the hau CMS sterile line. Western blotting analysis showed that this predicted open reading frame (ORF) was translated in the mitochondria of male-sterile plants. Furthermore, the growth of Escherichia coli was significantly repressed in the presence of ORF288, which indicated that this protein is toxic to the E. coli host cells. To confirm further the function of orf288 in male sterility, the gene was fused to a mitochondrial-targeting pre-sequence under the control of the Arabidopsis APETALA3 promoter and introduced into Arabidopsis thaliana. Almost 80% of transgenic plants with orf288 failed to develop anthers. It was also found that the independent expression of orf288 caused male sterility in transgenic plants, even without the transit pre-sequence. Furthermore, transient expression of orf288 and green fluorescent protein (GFP) as a fused protein in A. thaliana protoplasts showed that ORF288 was able to anchor to mitochondria even without the external mitochondrial-targeting peptide. These observations provide important evidence that orf288 is responsible for the male sterility of hau CMS in Brassica juncea.
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