ArticleJournal of clinical microbiology2012
Development of a multiplex PCR assay for detection and genogrouping of Neisseria meningitidis.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
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- Cross-reactivity of 4CMenB vaccine-induced antibodies against meningococci belonging to non-B serogroups in Italy.Human vaccines & immunotherapeutics · 2021Article
- A Narrative Review of the Molecular Epidemiology and Laboratory Surveillance of Vaccine Preventable Bacterial Meningitis Agents:Microorganisms · 2021Review
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- Meningococcal B vaccine antigen FHbp variants among disease-causing Neisseria meningitidis B isolates, Italy, 2014-2017.PloS one · 2020Article
- Filamentation initiated by Cas2 and its association with the acquisition process in cells.International journal of oral science · 2019Article
- Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detectInfection and drug resistance · 2019Article
- Neisseria meningitidis Antimicrobial Resistance in Italy, 2006 to 2016.Antimicrobial agents and chemotherapy · 2018Article
- Invasive meningococcal disease in Shanghai, China from 1950 to 2016: implications for serogroup B vaccine implementation.Scientific reports · 2018Article
- Development of a PCR algorithm to detect and characterize Neisseria meningitidis carriage isolates in the African meningitis belt.PloS one · 2018Article
- Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXIranian biomedical journal · 2017Article
- Characterization and Distribution of theFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neisseria meningitidis is a leading pathogen of epidemic bacterial meningitis and fulminant sepsis worldwide. Twelve different N. meningitidis serogroups have been identified to date based on antigenic differences in the capsular polysaccharide. However, more than 90% of human cases of N. meningitidis meningitis are the result of infection with just five serogroups, A, B, C, W135, and Y. Efficient methods of detection and genogrouping of N. meningitidis isolates are needed, therefore, in order to monitor prevalent serogroups as a means of disease control and prevention. The capsular gene complex regions have been sequenced from only seven out of the 12 serogroups. In this study, the capsular gene complexes of the remaining five serogroups were sequenced and analyzed. Primers were designed that were specific for N. meningitidis species and for the 12 individual serogroups, and a multiplex PCR assay using these specific primers was developed for N. meningitidis detection and genogrouping. The assay was tested using 15 reference strains covering all 12 serogroups, 143 clinical isolates, and 21 strains from closely related species or from species that cause meningitis. The assay could detect N. meningitidis serogroups and was shown to be specific, with a detection sensitivity of 1 ng of genomic DNA (equivalent to ∼4 × 10(5) genomes) or 3 × 10(5) CFU/ml in noncultured mock cerebrospinal fluid (CSF) specimens. This study, therefore, describes for the first time the development of a molecular protocol for the detection of all N. meningitidis serogroups. This multiplex PCR-based assay may have use for the clinical diagnosis and epidemiological surveillance of N. meningitidis.
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