ArticleGenome research2012
Developmental control of gene copy number by repression of replication initiation and fork progression.
Article in Genome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Article
- Super-resolution microscopy reveals stochastic initiation of replication in Drosophila polytene chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Article
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- Replication timing analysis in polyploid cells reveals Rif1 uses multiple mechanisms to promote underreplication in Drosophila.Genetics · 2021Article
- Article
- Article
- Effects of Mutations in theCells · 2020Article
- Rif1 Functions in a Tissue-Specific Manner To Control Replication Timing Through Its PP1-Binding Motif.Genetics · 2020Article
- Article
- Faint gray bands in Drosophila melanogaster polytene chromosomes are formed by coding sequences of housekeeping genes.Chromosoma · 2020Article
- Flying High-Muscle-Specific Underreplication inGenes · 2020Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster.Chromosoma · 2019Article
- Differences in firing efficiency, chromatin, and transcription underlie the developmental plasticity of theGenome research · 2019Article
- Article
- Genetic Organization of Open Chromatin Domains Situated in Polytene Chromosome Interbands in Drosophila.Doklady. Biochemistry and biophysics · 2018Article
- Article
- Dynamic changes in ORC localization and replication fork progression during tissue differentiation.BMC genomics · 2018Article
- Polytene Chromosomes - A Portrait of Functional Organization of theCurrent genomics · 2018Review
- Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
Precise DNA replication is crucial for genome maintenance, yet this process has been inherently difficult to study on a genome-wide level in untransformed differentiated metazoan cells. To determine how metazoan DNA replication can be repressed, we examined regions selectively under-replicated in Drosophila polytene salivary glands, and found they are transcriptionally silent and enriched for the repressive H3K27me3 mark. In the first genome-wide analysis of binding of the origin recognition complex (ORC) in a differentiated metazoan tissue, we find that ORC binding is dramatically reduced within these large domains, suggesting reduced initiation as one mechanism leading to under-replication. Inhibition of replication fork progression by the chromatin protein SUUR is an additional repression mechanism to reduce copy number. Although repressive histone marks are removed when SUUR is mutated and copy number restored, neither transcription nor ORC binding is reinstated. Tethering of the SUUR protein to a specific site is insufficient to block replication, however. These results establish that developmental control of DNA replication, at both the initiation and elongation stages, is a mechanism to change gene copy number during differentiation.
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