ArticleMolecular biology of the cell2012
Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Meiosis II spindle disassembly requires two distinct pathways.Molecular biology of the cell · 2023Article
- Atomic force microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly.The Journal of cell biology · 2018Article
- Article
- Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.PLoS genetics · 2016Article
- Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.The Journal of cell biology · 2015Article
- Role and regulation of kinesin-8 motors through the cell cycle.Systems and synthetic biology · 2014Article
- Cell cycle-dependent regulation of the RNA-binding protein Staufen1.Nucleic acids research · 2014Article
- An array of nuclear microtubules reorganizes the budding yeast nucleus during quiescence.The Journal of cell biology · 2013Article
- The multiple talents of kinesin-8.Nature cell biology · 2013Article
- Move in for the kill: motile microtubule regulators.Trends in cell biology · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Incomplete mitotic spindle disassembly causes lethality in budding yeast. To determine why spindle disassembly is required for cell viability, we used live-cell microscopy to analyze a double mutant strain containing a conditional mutant and a deletion mutant compromised for the kinesin-8 and anaphase-promoting complex-driven spindle-disassembly pathways (td-kip3 and doc1Δ, respectively). Under nonpermissive conditions, spindles in td-kip3 doc1Δ cells could break apart but could not disassemble completely. These cells could exit mitosis and undergo cell division. However, the daughter cells could not assemble functional, bipolar spindles in the ensuing mitosis. During the formation of these dysfunctional spindles, centrosome duplication and separation, as well as recruitment of key midzone-stabilizing proteins all appeared normal, but microtubule polymerization was nevertheless impaired and these spindles often collapsed. Introduction of free tubulin through episomal expression of α- and β-tubulin or introduction of a brief pulse of the microtubule-depolymerizing drug nocodazole allowed spindle assembly in these td-kip3 doc1Δ mutants. Therefore we propose that spindle disassembly is essential for regeneration of the intracellular pool of assembly-competent tubulin required for efficient spindle assembly during subsequent mitoses of daughter cells.
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Registered trials
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