ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Heterologous expression of functional G-protein-coupled receptors in Caenorhabditis elegans.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A short story on how chromophore is hydrolyzed from rhodopsin for recycling.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Review
- Identification, tissue specific expression analysis and functional characterization of arrestin gene (ARRDC) in the earthworm Eudrilus eugeniae: a molecular hypothesis behind worm photoreception.Molecular biology reports · 2022Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Genetic behavioral screen identifies an orphan anti-opioid system.Science (New York, N.Y.) · 2019Article
- Article
- Deciphering and modulating G protein signalling in C. elegans using the DREADD technology.Scientific reports · 2016Article
- Reciprocal Changes in Phosphoenolpyruvate Carboxykinase and Pyruvate Kinase with Age Are a Determinant of Aging in Caenorhabditis elegans.The Journal of biological chemistry · 2016Article
- Isotopic labeling of mammalian G protein-coupled receptors heterologously expressed in Caenorhabditis elegans.Analytical biochemistry · 2015Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- The G protein-coupled receptor rhodopsin: a historical perspective.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Inherent instability of the retinitis pigmentosa P23H mutant opsin.The Journal of biological chemistry · 2014Article
- From atomic structures to neuronal functions of g protein-coupled receptors.Annual review of neuroscience · 2013Review
- Expression of mammalian G protein-coupled receptors in Caenorhabditis elegans.Methods in enzymology · 2013Article
- Light-sensitive coupling of rhodopsin and melanopsin to G(i/o) and G(q) signal transduction in Caenorhabditis elegans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
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8 authors.
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Abstract
New strategies for expression, purification, functional characterization, and structural determination of membrane-spanning G-protein-coupled receptors (GPCRs) are constantly being developed because of their importance to human health. Here, we report a Caenorhabditis elegans heterologous expression system able to produce milligram amounts of functional native and engineered GPCRs. Both bovine opsin [(b)opsin] and human adenosine A(2A) subtype receptor [(h)A(2A)R] expressed in neurons or muscles of C. elegans were localized to cell membranes. Worms expressing these GPCRs manifested changes in motor behavior in response to light and ligands, respectively. With a newly devised protocol, 0.6-1 mg of purified homogenous 9-cis-retinal-bound bovine isorhodopsin [(b)isoRho] and ligand-bound (h)A(2A)R were obtained from C. elegans from one 10-L fermentation at low cost. Purified recombinant (b)isoRho exhibited its signature absorbance spectrum and activated its cognate G-protein transducin in vitro at a rate similar to native rhodopsin (Rho) obtained from bovine retina. Generally high expression levels of 11 native and mutant GPCRs demonstrated the potential of this C. elegans system to produce milligram quantities of high-quality GPCRs and possibly other membrane proteins suitable for detailed characterization.
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