ArticlePlant molecular biology2012
Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: lessons from transformation assays and genome-wide expression profiling.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 63 citations in OpenAlex.
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- In Vitro Induction of Interspecific Hybrid and Polyploidy Derived fromPlants (Basel, Switzerland) · 2023Article
- An Efficient Transformation System for Fast Production ofPlants (Basel, Switzerland) · 2023Article
- CRISPR-Mediated Base Editing: From Precise Point Mutation to Genome-Wide Engineering in Nonmodel Microbes.Biology · 2022Review
- Comparative transcriptome analysis reveals compatible and recalcitrant genotypic response of barley microspore-derived embryogenic callus toward Agrobacterium infection.BMC plant biology · 2021Article
- Transient expression of thePlant signaling & behavior · 2020Article
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- An Agrobacterium tumefaciens Strain with Gamma-Aminobutyric Acid Transaminase Activity Shows an Enhanced Genetic Transformation Ability in Plants.Scientific reports · 2017Article
- Transcriptomic and Hormonal Analyses Reveal that YUC-Mediated Auxin Biogenesis Is Involved in Shoot Regeneration from Rhizome inFrontiers in plant science · 2017Article
- Functional classification of rice flanking sequence tagged genes using MapMan terms and global understanding on metabolic and regulatory pathways affected by dxr mutant having defects in light response.Rice (New York, N.Y.) · 2016Article
- Unmasking host and microbial strategies in the Agrobacterium-plant defense tango.Frontiers in plant science · 2015Review
- TaWRKY68 responses to biotic stresses are revealed by the orthologous genes from major cereals.Genetics and molecular biology · 2014Article
- Agrobacterium infection and plant defense-transformation success hangs by a thread.Frontiers in plant science · 2013Review
- An improved Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.) cultivars.Protoplasma · 2013Article
- Identification of QTLs associated with tissue culture response through sequencing-based genotyping of RILs derived from 93-11 × Nipponbare in rice (Oryza sativa).Plant cell reports · 2013Article
- Global analysis of differentially expressed genes and proteins in the wheat callus infected by Agrobacterium tumefaciens.PloS one · 2013Article
- The role of the ubiquitin-proteasome system in Agrobacterium tumefaciens-mediated genetic transformation of plants.Plant physiology · 2012Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens-mediated genetic transformation has been routinely used in rice for more than a decade. However, the transformation efficiency of the indica rice variety is still unsatisfactory and much lower than that of japonica cultivars. Further improvement on the transformation efficiency lies in the genetic manipulation of the plant itself, which requires a better understanding of the underlying process accounting for the susceptibility of plant cells to Agrobacterium infection as well as the identification of plant genes involved in the transformation process. In this study, transient and stable transformation assays using different japonica and indica cultivars showed that the lower transformation efficiency in indica rice was mainly due to the low efficiency in T-DNA integration into the plant genome. Analyses of the global gene expression patterns across the transformation process in different varieties revealed major differences in the expression of genes responding to Agrobacterium within the first 6 h after infection and more differentially expressed genes were observed in the indica cultivar Zhenshan 97 (ZS), with a number of genes repressed early during infection. Microarray analysis revealed an important effect of plant defense response on Agrobacterium-mediated transformation. It has been shown that some genes which may be necessary for the transformation process were down-regulated in the indica cultivar ZS. This dataset provided a versatile resource for plant genomic research to understand the regulatory network of transformation process, and showed great promise for improving indica rice transformation using genetic manipulation of the rice genome.
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