ArticleGene therapy2012
Suppression of collagen-induced arthritis by intra-articular lentiviral vector-mediated delivery of Toll-like receptor 7 short hairpin RNA gene.
Article in Gene therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- DLX4 regulates rheumatoid arthritis fibroblast-like synoviocytes invasiveness and a cancer transcriptomic signature.Scientific reports · 2025Article
- Exploring precision treatments in immune-mediated inflammatory diseases: Harnessing the infinite potential of nucleic acid delivery.Exploration (Beijing, China) · 2025Review
- Therapeutic Potential of Targeting the NLRP3 Inflammasome in Rheumatoid Arthritis.Inflammation · 2023Review
- Gene therapy approaches for equine osteoarthritis.Frontiers in veterinary science · 2022Review
- IRAK4 inhibition: a promising strategy for treating RA joint inflammation and bone erosion.Cellular & molecular immunology · 2021Article
- Article
- Contribution of Toll-Like Receptors and the NLRP3 Inflammasome in Rheumatoid Arthritis Pathophysiology.ImmunoTargets and therapy · 2021Review
- Gene Delivery to Joints by Intra-Articular Injection.Human gene therapy · 2018Review
- TLRs, future potential therapeutic targets for RA.Autoimmunity reviews · 2017Review
- Article
- MicroRNA-34a affects chondrocyte apoptosis and proliferation by targeting the SIRT1/p53 signaling pathway during the pathogenesis of osteoarthritis.International journal of molecular medicine · 2016Article
- Identification of a Novel Toll-like Receptor 7 Endogenous Ligand in Rheumatoid Arthritis Synovial Fluid That Can Provoke Arthritic Joint Inflammation.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- Construction and identification of an RNA interference lentiviral vector targeting the mouse TNF-α gene.Experimental and therapeutic medicine · 2015Article
- Promotion of Inflammatory Arthritis by Interferon Regulatory Factor 5 in a Mouse Model.Arthritis & rheumatology (Hoboken, N.J.) · 2015Article
- Gene Therapy for Autoimmune Disease.Clinical reviews in allergy & immunology · 2015Review
- Pattern recognition receptors as potential therapeutic targets in inflammatory rheumatic disease.Arthritis research & therapy · 2015Review
- Emerging role of endosomal toll-like receptors in rheumatoid arthritis.Frontiers in immunology · 2014Review
- Analyses of synovial tissues from arthritic and protected congenic rat strains reveal a new core set of genes associated with disease severity.Physiological genomics · 2013Article
- Lentiviral small hairpin RNA knockdown of macrophage inflammatory protein-1γ ameliorates experimentally induced osteoarthritis in mice.Human gene therapy · 2013Article
- Which form of collagen is suitable for nerve cell culture?Neural regeneration research · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knockdown of Toll-like receptors (TLRs) is a novel therapeutic strategy in treating patients with rheumatoid arthritis (RA). We examined the effects of lentiviral vector-mediated delivery of TLR7 short hairpin RNA gene (Lt.shTLR7) on collagen-induced arthritis (CIA). After being immunized on days 0 and 7, Sprague-Dawley rats received intra-articular (i.a.) injection of Lt.shTLR7 or scramble control vector on days 7 and 10. The therapeutic effects were evaluated by measuring ankle circumferences, articular index, and radiographic and histological scores on killing on day 16. Microvessel densities, vascular endothelial growth factor (VEGF) levels, pro-inflammatory cytokine concentrations and T-cell numbers within the synovial tissues were measured. Moreover, VEGF and pro-inflammatory cytokine concentrations in culture supernatants from TLR7-transfected synovial fibroblasts (SFs) stimulated with imiquimod or endogenous ligands were examined. There were significant reduction in ankle circumferences, articular indexes, and radiographic and histological scores. Microvessel densities, VEGF concentrations, interleukin (IL)-1β and IL-6 levels and T-cell densities within synovial tissues were significantly lower. Induction of VEGF, IL-1β and IL-6 production from stimulated SFs was significantly suppressed. Taken together, these data demonstrate the effects of i.a. lentiviral vector-mediated delivery of shTLR7 RNA gene on inhibition of CIA, and implicate the manipulation of TLR7 as a potential therapeutic strategy in RA patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.