ArticleDNA repair2012
Electron microscopy visualization of DNA-protein complexes formed by Ku and DNA ligase IV.
Article in DNA repair, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- Single-molecule techniques in studying the molecular mechanisms of DNA synapsis in non-homologous end-joining repair.Biophysics reports · 2025Article
- Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.G3 (Bethesda, Md.) · 2021Article
- X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology.Protein science : a publication of the Protein Society · 2021Review
- Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry.Nature communications · 2020Article
- What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models.Journal of chemical information and modeling · 2020Article
- The deubiquitinase OTUD5 regulates Ku80 stability and non-homologous end joining.Cellular and molecular life sciences : CMLS · 2019Article
- An Intrinsically Disordered APLF Links Ku, DNA-PKcs, and XRCC4-DNA Ligase IV in an Extended Flexible Non-homologous End Joining Complex.The Journal of biological chemistry · 2016Article
- Consider the workhorse: Nonhomologous end-joining in budding yeast.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Interplay between Ku and Replication Protein A in the Restriction of Exo1-mediated DNA Break End Resection.The Journal of biological chemistry · 2015Article
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- Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity.PLoS genetics · 2013Article
- Human LIGIV is synthetically lethal with the loss of Rad54B-dependent recombination and is required for certain chromosome fusion events induced by telomere dysfunction.Nucleic acids research · 2013Article
- C-terminal low-complexity sequence repeats of Mycobacterium smegmatis Ku modulate DNA binding.Bioscience reports · 2013Article
- A noncatalytic function of the ligation complex during nonhomologous end joining.The Journal of cell biology · 2013Article
- Multiple protein-protein interactions within the DNA-PK complex are mediated by the C-terminus of Ku 80.International journal of biochemistry and molecular biology · 2012Article
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Authors and funding
7 authors.
Funding
Abstract
The repair of DNA double-stranded breaks (DSBs) is essential for cell viability and genome stability. Aberrant repair of DSBs has been linked with cancer predisposition and aging. During the repair of DSBs by non-homologous end joining (NHEJ), DNA ends are brought together, processed and then joined. In eukaryotes, this repair pathway is initiated by the binding of the ring-shaped Ku heterodimer and completed by DNA ligase IV. The DNA ligase IV complex, DNA ligase IV/XRRC4 in humans and Dnl4/Lif1 in yeast, is recruited to DNA ends in vitro and in vivo by an interaction with Ku and, in yeast, Dnl4/Lif1 stabilizes the binding of yKu to in vivo DSBs. Here we have analyzed the interactions of these functionally conserved eukaryotic NHEJ factors with DNA by electron microscopy. As expected, the ring-shaped Ku complex bound stably and specifically to DNA ends at physiological salt concentrations. At a ratio of 1 Ku molecule per DNA end, the majority of DNA ends were occupied by a single Ku complex with no significant formation of linear DNA multimers or circular loops. Both Dnl4/Lif1 and DNA ligase IV/XRCC4 formed complexes with Ku-bound DNA ends, resulting in intra- and intermolecular DNA end bridging, even with non-ligatable DNA ends. Together, these studies, which provide the first visualization of the conserved complex formed by Ku and DNA ligase IV at juxtaposed DNA ends by electron microscopy, suggest that the DNA ligase IV complex mediates end-bridging by engaging two Ku-bound DNA ends.
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