ArticlePLoS neglected tropical diseases2011
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 67 citations in OpenAlex.
- Prevalence and risk factors related toOne health (Amsterdam, Netherlands) · 2026Article
- Hepatobiliary fascioliasis: A neglected re-emerging threat, its diagnostic and management challenges.World journal of gastrointestinal pathophysiology · 2025Review
- Lateral-Flow Antibody Detection Device for the Diagnosis of Human Fascioliasis Using Simulated Whole-Blood Samples.The American journal of tropical medicine and hygiene · 2025Article
- Diagnosis of fasciolosis antibodies in Brazilian cattle through ELISA employing both native and recombinant antigens.Microbiology spectrum · 2024Article
- Increased specificity of Fasciola hepatica excretory-secretory antigens combining negative selection on hydroxyapatite and salt precipitation.Scientific reports · 2024Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Fascioliasis.Advances in experimental medicine and biology · 2024Article
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- Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes.Biosensors · 2022Article
- High relatedness of bioinformatic data and realistic experimental works on the potentials ofFrontiers in public health · 2022Article
- Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep.PloS one · 2022Article
- Pathogenicity and virulence of the liver flukesVirulence · 2021Article
- Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.Acta parasitologica · 2021Review
- Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages.Sensors (Basel, Switzerland) · 2020Article
- Effectiveness of Fasciola gigantica excretory-secretory and recombinant cathepsin L antigens for rapid diagnosis of human fascioliasis using immunochromatographic devices.Parasitology research · 2020Article
- Pilot Evaluation of TwoMolecules (Basel, Switzerland) · 2020Article
- Drug resistance in liver flukes.International journal for parasitology. Drugs and drug resistance · 2020Review
- Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.Journal of advanced veterinary and animal research · 2019Article
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Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. METHODOLOGY/PRINCIPAL
findingsWe have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. CONCLUSIONS/SIGNIFICANCE: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.