ArticlePLoS neglected tropical diseases2011
Comparison of two multilocus sequence based genotyping schemes for Leptospira species.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epidemiology of Leptospirosis in Africa: A Systematic Review of a Neglected Zoonosis and a Paradigm for 'One Health' in Africa.PLoS neglected tropical diseases · 2015Pooled it
- Molecular epidemiology of pathogenic Leptospira spp. in Thailand during 2024-2025, with detection of Leptospira weilii in human clinical specimens.PLoS neglected tropical diseases · 2026Article
- Molecular Detection and Genotyping of PathogenicMicroorganisms · 2026Article
- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. Using the Bacterial Isolate Genome Sequence Database.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Host-Driven Genetic Diversity ofTransboundary and emerging diseases · 2026Article
- Shaping the future ofJournal of medical microbiology · 2025Review
- Detection and Identification of PathogenicTransboundary and emerging diseases · 2024Article
- Utility evaluation of two molecular methods forHeliyon · 2023Article
- Application of Multilocus Sequence Typing for the Characterization ofTropical medicine and infectious disease · 2023Article
- Occurrence of Bacterial and Protozoan Pathogens in Red Foxes (Animals : an open access journal from MDPI · 2022Article
- Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain.PLoS neglected tropical diseases · 2021Article
- Genus-wide Leptospira core genome multilocus sequence typing for strain taxonomy and global surveillance.PLoS neglected tropical diseases · 2019Article
- Isolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis.PLoS neglected tropical diseases · 2018Article
- Evidence of infection with Leptospira interrogans and spotted fever group rickettsiae among rodents in an urban area of Osaka City, Japan.The Journal of veterinary medical science · 2017Article
- Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains.Applied and environmental microbiology · 2017Article
- Culture Strategies for Isolation of FastidiousFrontiers in microbiology · 2017Article
- Identification of Tenrec ecaudatus, a Wild Mammal Introduced to Mayotte Island, as a Reservoir of the Newly Identified Human Pathogenic Leptospira mayottensis.PLoS neglected tropical diseases · 2016Article
- What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus Leptospira.PLoS neglected tropical diseases · 2016Article
- Assessment of two multilocus sequence typing (MLST) schemes available for Streptococcus mutans.Archives of oral biology · 2015Article
- Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
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Abstract
backgroundSeveral sequence based genotyping schemes have been developed for Leptospira spp. The objective of this study was to genotype a collection of clinical and reference isolates using the two most commonly used schemes and compare and contrast the results. METHODS AND
findingsA total of 48 isolates consisting of L. interrogans (n = 40) and L. kirschneri (n = 8) were typed by the 7 locus MLST scheme described by Thaipadungpanit et al., and the 6 locus genotyping scheme described by Ahmed et al., (termed 7L and 6L, respectively). Two L. interrogans isolates were not typed using 6L because of a deletion of three nucleotides in lipL32. The remaining 46 isolates were resolved into 21 sequence types (STs) by 7L, and 30 genotypes by 6L. Overall nucleotide diversity (based on concatenated sequence) was 3.6% and 2.3% for 7L and 6L, respectively. The D value (discriminatory ability) of 7L and 6L were comparable, i.e. 92.0 (95% CI 87.5-96.5) vs. 93.5 (95% CI 88.6-98.4). The dN/dS ratios calculated for each locus indicated that none were under positive selection. Neighbor joining trees were reconstructed based on the concatenated sequences for each scheme. Both trees showed two distinct groups corresponding to L. interrogans and L. kirschneri, and both identified two clones containing 10 and 7 clinical isolates, respectively. There were six instances in which 6L split single STs as defined by 7L into closely related clusters. We noted two discrepancies between the trees in which the genetic relatedness between two pairs of strains were more closely related by 7L than by 6L.
conclusionsThis genetic analysis indicates that the two schemes are comparable. We discuss their practical advantages and disadvantages.
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