ArticlePloS one2011
Innate host response in primary human hepatocytes with hepatitis C virus infection.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- CASP-Model Sepsis Triggers Systemic Innate Immune Responses Revealed by the Systems-Level Signaling Pathways.Frontiers in immunology · 2022Article
- The Dark Side of the Force: When the Immune System Is the Fuel of Tumor Onset.International journal of molecular sciences · 2021Review
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- Effect of P-body component Mov10 on HCV virus production and infectivity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Role of S100A3 in human hepatocellular carcinoma and the anticancer effect of sodium cantharidinate.Experimental and therapeutic medicine · 2017Article
- Chronic hepatitis C virus infection and pathogenesis of hepatocellular carcinoma.Current opinion in virology · 2016Review
- Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies.Virology · 2016Article
- Interplay between Inflammation and Cellular Stress Triggered by Flaviviridae Viruses.Frontiers in microbiology · 2016Review
- Hepatitis C virus and antiviral innate immunity: who wins at tug-of-war?World journal of gastroenterology · 2015Review
- Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Inhibition of hepatitis C virus production by aptamers against the core protein.Journal of virology · 2014Article
- Primary hepatocytes and their cultures in liver apoptosis research.Archives of toxicology · 2014Review
- Article
- Article
- Inhibition of hepatitis C virus infection by DNA aptamer against envelope protein.Antimicrobial agents and chemotherapy · 2013Article
- 2-octynoic acid inhibits hepatitis C virus infection through activation of AMP-activated protein kinase.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
BACKGROUND AND
aimThe interaction between hepatitis C virus (HCV) and innate antiviral defense systems in primary human hepatocytes is not well understood. The objective of this study is to examine how primary human hepatocytes response to HCV infection.
methodsAn infectious HCV isolate JFH1 was used to infect isolated primary human hepatocytes. HCV RNA or NS5A protein in the cells was detected by real-time PCR or immunofluorescence staining respectively. Apoptosis was examined with flow cytometry. Mechanisms of HCV-induced IFN-β expression and apoptosis were determined.
resultsPrimary human hepatocytes were susceptible to JFH1 virus and released infectious virus. IFN-α inhibited viral RNA replication in the cells. IFN-β and interferon-stimulated genes were induced in the cells during acute infection. HCV infection induced apoptosis of primary human hepatocytes through the TRAIL-mediated pathway. Silencing RIG-I expression in primary human hepatocytes inhibited IFN-β and TRAIL expression and blocked apoptosis of the cells, which facilitated viral RNA replication in the cells. Moreover, HCV NS34A protein inhibited viral induced IFN-β expression in primary human hepatocytes.
conclusionInnate host response is intact in HCV-infected primary human hepatocytes. RIG-I plays a key role in the induction of IFN and TRAIL by viruses and apoptosis of primary human hepatocytes via activation of the TRAIL-mediated pathway. HCV NS34A protein appears to be capable of disrupting the innate antiviral host responses in primary human hepatocytes. Our study provides a novel mechanism by which primary human hepatocytes respond to natural HCV infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.