ArticlePloS one2011
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
88 citing papers in PubMed, 1 synthesis or guideline pooled it, 220 citations in OpenAlex.
- Evaluating the efficacy of human dental pulp stem cells and scaffold combination for bone regeneration in animal models: a systematic review and meta-analysis.Stem cell research & therapy · 2023Pooled it
- Chemically Defined Medium Enables GDNF-Driven Early Neuronal-like Phenotype of Human Dental Pulp Stem Cells.Cells · 2026Article
- SHEDs and BMSCs exhibit distinct lineage preferences in HUVECs dynamic spheroid co-cultures: vascular versus osteogenic commitment.Bioengineering & translational medicine · 2026Article
- Research progress in heterogeneity of dental mesenchymal stem cells.International journal of oral science · 2026Review
- Crosstalk Between Hematopoietic and Mesenchymal Cells in Bone and Tooth Immune Response.Advances in experimental medicine and biology · 2026Review
- Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources.Journal of translational medicine · 2025Review
- Conditioned media of stem cells from human exfoliated deciduous teeth contain factors related to extracellular matrix organization and promotes corneal epithelial wound healing.Regenerative therapy · 2025Article
- Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren's Syndrome: A Preclinical Study.Biomedicines · 2025Article
- Human dental pulp stem cells for spinal cord injury.Stem cell research & therapy · 2025Review
- PDGF-BB overexpressing dental pulp stem cells improve angiogenesis in dental pulp regeneration.Frontiers in bioengineering and biotechnology · 2025Article
- Zirconia/dental pulp stem cell composite scaffolds repair osteogenic defect via regulating macrophages.Frontiers in bioengineering and biotechnology · 2025Article
- Craniofacial chondrogenesis in organoids from human stem cell-derived neural crest cells.iScience · 2024Article
- Effect of Octamer-Binding Transcription Factor 4 Overexpression on the Neural Induction of Human Dental Pulp Stem Cells.Stem cell reviews and reports · 2024Article
- Article
- Dentin matrix protein 1 and HUVEC-ECM scaffold promote the differentiation of human dental pulp stem cells into endothelial lineage: implications in regenerative medicine.Frontiers in physiology · 2024Article
- Research progress in cell therapy for oral diseases: focus on cell sources and strategies to optimize cell function.Frontiers in bioengineering and biotechnology · 2024Review
- Potential Application of Orofacial MSCs in Tissue Engineering Nerve Guidance for Peripheral Nerve Injury Repair.Stem cell reviews and reports · 2023Review
- Potential of dental pulp stem cells and their products in promoting peripheral nerve regeneration and their future applications.World journal of stem cells · 2023Review
- Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells.BMC veterinary research · 2023Article
- A New Landscape of Human Dental Aging: Causes, Consequences, and Intervention Avenues.Aging and disease · 2023Review
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Dental pulp stem cells (DPSCs) are shown to reside within the tooth and play an important role in dentin regeneration. DPSCs were first isolated and characterized from human teeth and most studies have focused on using this adult stem cell for clinical applications. However, mouse DPSCs have not been well characterized and their origin(s) have not yet been elucidated. Herein we examined if murine DPSCs are neural crest derived and determined their in vitro and in vivo capacity. DPSCs from neonatal murine tooth pulp expressed embryonic stem cell and neural crest related genes, but lacked expression of mesodermal genes. Cells isolated from the Wnt1-Cre/R26R-LacZ model, a reporter of neural crest-derived tissues, indicated that DPSCs were Wnt1-marked and therefore of neural crest origin. Clonal DPSCs showed multi-differentiation in neural crest lineage for odontoblasts, chondrocytes, adipocytes, neurons, and smooth muscles. Following in vivo subcutaneous transplantation with hydroxyapatite/tricalcium phosphate, based on tissue/cell morphology and specific antibody staining, the clones differentiated into odontoblast-like cells and produced dentin-like structure. Conversely, bone marrow stromal cells (BMSCs) gave rise to osteoblast-like cells and generated bone-like structure. Interestingly, the capillary distribution in the DPSC transplants showed close proximity to odontoblasts whereas in the BMSC transplants bone condensations were distant to capillaries resembling dentinogenesis in the former vs. osteogenesis in the latter. Thus we demonstrate the existence of neural crest-derived DPSCs with differentiation capacity into cranial mesenchymal tissues and other neural crest-derived tissues. In turn, DPSCs hold promise as a source for regenerating cranial mesenchyme and other neural crest derived tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.