ArticleNucleic acids research2012
Direct, genome-wide assessment of DNA mutations in single cells.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 75 citations in OpenAlex.
- Somatic mutations and genome mosaicism in aging and disease.Experimental & molecular medicine · 2026Review
- Single-Cell Genomics and Somatic Variation in Circulating and Cardiac Resident Cells.Circulation research · 2026Review
- Double strand breaks drive toxicity in Huntington's disease mice with or without somatic expansion.bioRxiv : the preprint server for biology · 2025Article
- Methods and applications of genome-wide profiling of DNA damage and rare mutations.Nature reviews. Genetics · 2024Review
- Somatic mutations in aging and disease.GeroScience · 2024Review
- Concurrent analysis of genome and transcriptome in one single cell.BMC research notes · 2024Article
- Analyzing somatic mutations by single-cell whole-genome sequencing.Nature protocols · 2024Review
- Somatic mutation burden in relation to aging and functional life span: implications for cellular reprogramming and rejuvenation.Current opinion in genetics & development · 2023Review
- Article
- Single-molecule, quantitative detection of low-abundance somatic mutations by high-throughput sequencing.Science advances · 2022Article
- Age-related somatic mutation burden in human tissues.Frontiers in aging · 2022Review
- From DNA damage to mutations: All roads lead to aging.Ageing research reviews · 2021Review
- The genomes of precision edited cloned calves show no evidence for off-target events or increased de novo mutagenesis.BMC genomics · 2021Article
- Accurate genomic variant detection in single cells with primary template-directed amplification.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Identification of Somatic Mutations From Bulk and Single-Cell Sequencing Data.Frontiers in aging · 2021Review
- Single-cell omics in ageing: a young and growing field.Nature metabolism · 2020Review
- Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver.Science advances · 2020Article
- SCCNV: A Software Tool for Identifying Copy Number Variation From Single-Cell Whole-Genome Sequencing.Frontiers in genetics · 2020Article
- Somatic mutation load and spectra: A record of DNA damage and repair in healthy human cells.Environmental and molecular mutagenesis · 2018Review
- Molecular Mechanisms Determining Lifespan in Short- and Long-Lived Species.Trends in endocrinology and metabolism: TEM · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DNA mutations are the inevitable consequences of errors that arise during replication and repair of DNA damage. Because of their random and infrequent occurrence, quantification and characterization of DNA mutations in the genome of somatic cells has been difficult. Random, low-abundance mutations are currently inaccessible by standard high-throughput sequencing approaches because they cannot be distinguished from sequencing errors. One way to circumvent this problem and simultaneously account for the mutational heterogeneity within tissues is whole genome sequencing of a representative number of single cells. Here, we show elevated mutation levels in single cells from Drosophila melanogaster S2 and mouse embryonic fibroblast populations after treatment with the powerful mutagen N-ethyl-N-nitrosourea. This method can be applied as a direct measure of exposure to mutagenic agents and for assessing genotypic heterogeneity within tissues or cell populations.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.