ArticleNucleic acids research2012
Do replication forks control late origin firing in Saccharomyces cerevisiae?
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Dual DNA replication modes: varying fork speeds and initiation rates within the spatial replication program in Xenopus.Nucleic acids research · 2025Article
- Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming.Nature communications · 2025Article
- Genome-wide mapping of individual replication fork velocities using nanopore sequencing.Nature communications · 2022Article
- FORK-seq: Single-Molecule Profiling of DNA Replication.Methods in molecular biology (Clifton, N.J.) · 2022Article
- FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing.Genome biology · 2020Article
- On the Interplay of the DNA Replication Program and the Intra-S Phase Checkpoint Pathway.Genes · 2019Review
- Deciphering DNA replication dynamics in eukaryotic cell populations in relation with their averaged chromatin conformations.Scientific reports · 2016Article
- Behavior of replication origins in Eukaryota - spatio-temporal dynamics of licensing and firing.Cell cycle (Georgetown, Tex.) · 2015Review
- Location, location, location: it's all in the timing for replication origins.Genes & development · 2013Review
- Back to the origin: reconsidering replication, transcription, epigenetics, and cell cycle control.Genes & cancer · 2012Article
- Mathematical modeling of genome replication.Physical review. E, Statistical, nonlinear, and soft matter physics · 2012Article
- Replication timing and its emergence from stochastic processes.Trends in genetics : TIG · 2012Review
- Evidence for sequential and increasing activation of replication origins along replication timing gradients in the human genome.PLoS computational biology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Recent studies of eukaryotic DNA replication timing profiles suggest that the time-dependent rate of origin firing, I(t), has a universal shape, which ensures a reproducible replication completion time. However, measurements of I(t) are based on population averages, which may bias the shape of the I(t) because of imperfect cell synchrony and cell-to-cell variability. Here, we measure the population-averaged I(t) profile from synchronized Saccharomyces cerevisiae cells using DNA combing and we extract the single-cell I(t) profile using numerical deconvolution. The single cell I(t) and the population-averaged I(t) extracted from DNA combing and replication timing profiles are similar, indicating a genome scale invariance of the replication process, and excluding cell-to-cell variability in replication time as an explanation for the shape of I(t). The single cell I(t) correlates with fork density in wild-type cells, which is specifically loosened in late S phase in the clb5Δ mutant. A previously proposed numerical model that reproduces the wild-type I(t) profile, could also describe the clb5Δ mutant I(t) once modified to incorporate the decline in CDK activity and the looser dependency of initiation on fork density in the absence of Clb5p. Overall, these results suggest that the replication forks emanating from early fired origins facilitate origin firing in later-replicating regions.
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