ArticleChemphyschem : a European journal of chemical physics and physical chemistry2012
Optical modulation and selective recovery of Cy5 fluorescence.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters.The journal of physical chemistry. B · 2024Article
- Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.The journal of physical chemistry. B · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Synchronously Amplified Photoacoustic Image Recovery (SAPhIRe).Photoacoustics · 2020Article
- Macroscale fluorescence imaging against autofluorescence under ambient light.Light, science & applications · 2018Article
- Optically Activated Delayed Fluorescence.The journal of physical chemistry letters · 2017Article
- Improved Fluorescent Protein Contrast and Discrimination by Optically Controlling Dark State Lifetimes.The journal of physical chemistry letters · 2017Article
- Dark State-Modulated Fluorescence Correlation Spectroscopy for Quantitative Signal Recovery.The journal of physical chemistry letters · 2016Article
- Optically Modulated Photoswitchable Fluorescent Proteins Yield Improved Biological Imaging Sensitivity.Journal of the American Chemical Society · 2015Article
- Expanding discriminative dimensions for analysis and imaging.Chemical science · 2015Article
- Tailoring cyanine dark states for improved optically modulated fluorescence recovery.The journal of physical chemistry. B · 2015Article
- Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background.Accounts of chemical research · 2014Review
- Optically modulatable blue fluorescent proteins.Journal of the American Chemical Society · 2013Article
- Modulated fluorophore signal recovery buried within tissue mimicking phantoms.The journal of physical chemistry. A · 2013Article
- Signal Discrimination Between Fluorescent Proteins in Live Cells by Long-wavelength Optical Modulation.The journal of physical chemistry letters · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence modulation offers the opportunity to detect low-concentration fluorophore signals within high background. Applicable from the single-molecule to bulk levels, we demonstrate long-wavelength optical depopulation of dark states that otherwise limit Cy5 fluorescence intensity. By modulated excitation of a long-wavelength Cy5 transient absorption, we dynamically modulate Cy5 emission. The frequency dependence enables specification of the dark-state timescales enabling optical-demodulation-based signal recovery from high background. These dual-laser illumination schemes for high-sensitivity fluorescence-signal recovery easily improve signal-to-noise ratios by well over an order of magnitude, largely by discrimination against background. Previously limited to very specialized dyes, our utilization of long-lived dark states in Cy5 enables selective detection of this very common single-molecule and bulk fluorophore. Although, in principle, the "dark state" can arise from any photoinduced process, we demonstrate that cis-trans photoisomerization, with its unique transient absorption and lifetime enables this sensitivity boosting, long-wavelength modulation to occur in Cy5. Such studies underscore the need for transient absorption studies on common fluorophores to extend the impact of fluorescence modulation for high-sensitivity fluorescence imaging in a much wider array of applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.