ArticleCancer prevention research (Philadelphia, Pa.)2012
N-nitroso-tris-chloroethylurea induces premalignant squamous dysplasia in mice.
Article in Cancer prevention research (Philadelphia, Pa.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Rho-associated kinase (ROCK)-associated proteins and genes-encoded proteins upregulated in lung squamous cell carcinoma (LUSC).Journal of the Egyptian National Cancer Institute · 2026Article
- Aberrant basal cell clonal dynamics shape early lung carcinogenesis.Science (New York, N.Y.) · 2025Article
- Article
- Pre-clinical lung squamous cell carcinoma mouse models to identify novel biomarkers and therapeutic interventions.Frontiers in oncology · 2023Review
- Primary epididymis squamous cell carcinoma in a CB6F1-Tg rasH2 mouse.Journal of toxicologic pathology · 2022Article
- Two cell line models to study multiorganic metastasis and immunotherapy in lung squamous cell carcinoma.Disease models & mechanisms · 2022Article
- NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model.Scientific reports · 2021Article
- An Improved Murine Premalignant Squamous Cell Model: Tobacco Smoke Exposure Augments NTCU-Induced Murine Airway Dysplasia.Cancer prevention research (Philadelphia, Pa.) · 2021Article
- Progress towards non-small-cell lung cancer models that represent clinical evolutionary trajectories.Open biology · 2021Review
- Two-stage 3-methylcholanthrene and butylated hydroxytoluene-induced lung carcinogenesis in mice.Methods in cell biology · 2021Article
- Histopathological effect of pterostilbene as chemoprevention in N-nitroso-tri-chloroethylurea (NTCU)-induced lung squamous cell carcinoma (SCC) mouse model.Histology and histopathology · 2020Article
- The Effect of Mouse Strain, Sex, and Carcinogen Dose on Toxicity and the Development of Lung Dysplasia and Squamous Cell Carcinomas in Mice.Cancer prevention research (Philadelphia, Pa.) · 2019Article
- Epithelial cell migration as a potential therapeutic target in early lung cancer.European respiratory review : an official journal of the European Respiratory Society · 2017Review
- Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.Cancer science · 2016Article
- Vitamin D Repletion Reduces the Progression of Premalignant Squamous Lesions in the NTCU Lung Squamous Cell Carcinoma Mouse Model.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Tracheal dysplasia precedes bronchial dysplasia in mouse model of N-nitroso trischloroethylurea induced squamous cell lung cancer.PloS one · 2015Article
- Dietary diindolylmethane suppresses inflammation-driven lung squamous cell carcinoma in mice.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Novel medium-term carcinogenesis model for lung squamous cell carcinoma induced by N-nitroso-tris-chloroethylurea in mice.Cancer science · 2013Article
- Lung cancer chemoprevention: current status and future prospects.Nature reviews. Clinical oncology · 2013Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Squamous cell carcinoma (SCC) and premalignant endobronchial lesions have been difficult to study in murine models. In this study, we evaluate the topical N-nitroso-tris-chloroethylurea (NTCU) murine SCC model, determine the extent to which resulting premalignant airway dysplasia develops, discuss clinicopathologic grading criteria in lesion progression, and confirm that immunohistochemical (IHC) staining patterns are consistent with those observed in human endobronchial dysplasia and SCC. Male and female FVB mice were treated biweekly with topical NTCU (4, 8, or 40 mmol/L) or vehicle for 32 weeks. Following sacrifice, squamous cell lesions were enumerated and categorized into the following groups: flat atypia, low-grade dysplasia, high-grade dysplasia, and invasive SCC. The 40 mmol/L NTCU concentration produced the entire spectrum of premalignant dysplasias and squamous cell carcinomas, but was associated with poor survival. Concentrations of 4 and 8 mmol/L NTCU were better tolerated and produced only significant levels of flat atypia. Squamous origin of the range of observed lesions was confirmed with IHC staining for cytokeratin 5/6, p63, thyroid transcription factor-1 (TTF-1), and Napsin-A. This study shows that topical application of high-dose NTCU produces endobronchial premalignant lesions with classic squamous characteristics and should allow for improved preclinical evaluation of potential chemopreventive agents.
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