ArticleMolecular therapy : the journal of the American Society of Gene Therapy2012
In vivo delivery of cytoplasmic RNA virus-derived miRNAs.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Translocating shRNA: a novel approach to RNA interference with Newcastle disease virus as viral vector.The Journal of general virology · 2025Article
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- Single-Dose Treatment With Vesicular Stomatitis Virus-Based Ebola Virus Vaccine Expressing Ebola Virus-Specific Artificial Micro-RNA Does Not Protect Mice From Lethal Disease.The Journal of infectious diseases · 2023Article
- Optimal delivery of RNA interference by viral vectors for cancer therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Review
- Article
- ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6.Viruses · 2022Article
- Virally programmed extracellular vesicles sensitize cancer cells to oncolytic virus and small molecule therapy.Nature communications · 2022Article
- A virus-derived microRNA targets immune response genes during SARS-CoV-2 infection.EMBO reports · 2022Article
- A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses.STAR protocols · 2020Article
- Ebola Virus Produces Discrete Small Noncoding RNAs Independently of the Host MicroRNA Pathway Which Lack RNA Interference Activity in Bat and Human Cells.Journal of virology · 2020Article
- A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs.Molecular therapy. Methods & clinical development · 2019Article
- MicroRNA-Attenuated Virus Vaccines.Non-coding RNA · 2018Review
- RNA virus building blocks-miRNAs not included.PLoS pathogens · 2018Article
- Virus-encoded miRNAs in Ebola virus disease.Scientific reports · 2018Article
- MicroRNA-Based Attenuation of Influenza Virus across Susceptible Hosts.Journal of virology · 2018Article
- Potential Role of MicroRNAs in the Regulation of Antiviral Responses to Influenza Infection.Frontiers in immunology · 2018Review
- Molecular mechanisms of Dicer: endonuclease and enzymatic activity.The Biochemical journal · 2017Review
- Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection.BMC genomics · 2017Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
The discovery of microRNAs (miRNAs) revealed an unappreciated level of post-transcriptional control used by the cell to maintain optimal protein levels. This process has represented an attractive strategy for therapeutics that is currently limited by in vivo delivery constraints. Here, we describe the generation of a single-stranded, cytoplasmic virus of negative polarity capable of producing functional miRNAs. Cytoplasmic RNA virus-derived miRNAs accumulated to high levels in vitro, generated significant amounts of miRNA star strand, associated with the RNA-induced silencing complex (RISC), and conferred post transcriptional gene silencing in a sequence-specific manner. Furthermore, we demonstrate that these vectors could deliver miRNAs to a wide range of tissues, and sustain prolonged expression capable of achieving measurable knockdown of physiological targets in vivo. Taken together, these results validate noncanonical processing of cytoplasmic-derived miRNAs and provide a novel platform for small RNA delivery.
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Registered trials
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