ArticleBMC microbiology2011
An alternative physiological role for the EmhABC efflux pump in Pseudomonas fluorescens cLP6a.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 54 citations in OpenAlex.
- Investigation of the effects of ampicillin and ceftazidime on Proteus mirabilis using metabolomic approaches and bioinformatics analyses.Folia microbiologica · 2026Article
- Efflux pump activation confers mupirocin resistance and enhances rhizosphere fitness inApplied and environmental microbiology · 2026Article
- Integrative genomic and transcriptomic insights into the biocontrol activity ofFrontiers in microbiology · 2026Article
- Effect of temperature on the activity of efflux pumps in selected species of human opportunistic bacterial pathogens.Memorias do Instituto Oswaldo Cruz · 2025Article
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- Involvement of the SCO3366 efflux pump from S. coelicolor in rifampicin resistance and its regulation by a TetR regulator.Applied microbiology and biotechnology · 2022Article
- Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.Chemical reviews · 2021Review
- Enrichment of antibiotic resistance genes (ARGs) in polyaromatic hydrocarbon-contaminated soils: a major challenge for environmental health.Environmental science and pollution research international · 2021Review
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- The Evolutionary Conservation of Escherichia coli Drug Efflux Pumps Supports Physiological Functions.Journal of bacteriology · 2020Review
- Antibiotic ResistantFoods (Basel, Switzerland) · 2019Review
- Identification of NovelmBio · 2019Article
- The influence of heavy metals, polyaromatic hydrocarbons, and polychlorinated biphenyls pollution on the development of antibiotic resistance in soils.Environmental science and pollution research international · 2018Article
- Efflux drug transporters at the forefront of antimicrobial resistance.European biophysics journal : EBJ · 2017Review
- Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 5: suitability of taxonomic units notified to EFSA until September 2016.EFSA journal. European Food Safety Authority · 2017Article
- Transcriptomic Analysis of Laribacter hongkongensis Reveals Adaptive Response Coupled with Temperature.PloS one · 2017Article
- Inducible Expression of a Resistance-Nodulation-Division-Type Efflux Pump in Staphylococcus aureus Provides Resistance to Linoleic and Arachidonic Acids.Journal of bacteriology · 2015Article
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- SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.PloS one · 2014Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEfflux pumps belonging to the resistance-nodulation-division (RND) superfamily in bacteria are involved in antibiotic resistance and solvent tolerance but have an unknown physiological role. EmhABC, a RND-type efflux pump in Pseudomonas fluorescens strain cLP6a, extrudes hydrophobic antibiotics, dyes and polycyclic aromatic hydrocarbons including phenanthrene. The effects of physico-chemical factors such as temperature or antibiotics on the activity and expression of EmhABC were determined in order to deduce its physiological role(s) in strain cLP6a in comparison to the emhB disruptant strain, cLP6a-1.
resultsEfflux assays conducted with (14)C-phenanthrene showed that EmhABC activity is affected by incubation temperature. Increased phenanthrene efflux was measured in cLP6a cells grown at 10°C and decreased efflux was observed at 35°C compared with cells grown at the optimum temperature of 28°C. Membrane fatty acids in cLP6a cells were substantially altered by changes in growth temperature and in the presence of tetracycline. Changed membrane fatty acids and increased membrane permeability were associated with ~30-fold increased expression of emhABC in cLP6a cells grown at 35°C, and with increased extracellular free fatty acids. Growth of P. fluorescens cLP6a at supra-optimal temperature was enhanced by the presence of EmhABC compared to strain cLP6a-1.
conclusionsCombined, these observations suggest that the EmhABC efflux pump may be involved in the management of membrane stress effects such as those due to unfavourable incubation temperatures. Efflux of fatty acids replaced as a result of membrane damage or phospholipid turnover may be the primary physiological role of the EmhABC efflux pump in P. fluorescens cLP6a.
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