Evidence map›Paper›PMID 22084416›Full record

ArticleThe Journal of general physiology2011

Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.

Grigory Maksaev, Elizabeth S Haswell

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of general physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Pressure and ultrasound activate mechanosensitive TRAAK KbioRxiv : the preprint server for biology · 2023
    Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Expressing and characterizing mechanosensitive channels in Xenopus oocytes.Methods in molecular biology (Clifton, N.J.) · 2015
    Article
  10. Article
  11. Article
  12. Family of prokaryote cyclic nucleotide-modulated ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2014
    Article
  13. Article
  14. Review
  15. Article
  16. MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions.Proceedings of the National Academy of Sciences of the United States of America · 2012
    Article
  17. Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Grigory MaksaevDepartment of Biology, Washington University in Saint Louis, Saint Louis, MO 63130, USA.
Elizabeth S Haswell
Washington University in St. Louis · US

Funding

Biophysical, Structural and Functional Analysis of Mechanosensitive ChannelsR01GM084211 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI HASWELL, ELIZABETH S, PHILLIPS, ROB · 2008 to 2016
$3.6M
NIGMS NIH HHS R01 GM084211NIGMS NIH HHS R01GM084211-01
6 · The paper itself

Abstract

We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.

Indexed as

AnimalsEscherichia coli ProteinsFemaleIon Channel GatingIon ChannelsMembrane PotentialsMicroscopy, ConfocalOocytesXenopus laevisEscherichia coli ProteinsIon ChannelsMscS protein, E coli

Identifiers

PMID22084416
PMCPMC3226970
OpenAlexW2126809427

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.