ArticleThe Journal of general physiology2011
Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.
Article in The Journal of general physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Tension activation of mechanosensitive two-pore domain K+ channels TRAAK, TREK-1, and TREK-2.Nature communications · 2024Article
- Pressure and ultrasound activate mechanosensitive TRAAK KbioRxiv : the preprint server for biology · 2023Article
- Interdependence of a mechanosensitive anion channel and glutamate receptors in distal wound signaling.Science advances · 2021Article
- Review
- Article
- Nonpolar residues in the presumptive pore-lining helix of mechanosensitive channel MSL10 influence channel behavior and establish a nonconducting function.Plant direct · 2018Article
- Mechanosensitive channel MSL8 regulates osmotic forces during pollen hydration and germination.Science (New York, N.Y.) · 2015Article
- United in diversity: mechanosensitive ion channels in plants.Annual review of plant biology · 2015Review
- Expressing and characterizing mechanosensitive channels in Xenopus oocytes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Mutations in a Conserved Domain of E. coli MscS to the Most Conserved Superfamily Residue Leads to Kinetic Changes.PloS one · 2015Article
- Arabidopsis MSL10 has a regulated cell death signaling activity that is separable from its mechanosensitive ion channel activity.The Plant cell · 2014Article
- Family of prokaryote cyclic nucleotide-modulated ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Voltage-dependent potassium currents expressed in Xenopus laevis oocytes after injection of mRNA isolated from trophozoites of Giardia lamblia (strain Portland-1).Physiological reports · 2013Article
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.Cold Spring Harbor protocols · 2013Article
- MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscL.Nature communications · 2012Article
- Recent characterizations of MscS and its homologs provide insight into the basis of ion selectivity in mechanosensitive channels.Channels (Austin, Tex.)Article
- Mechanical properties of lipid bilayers and regulation of mechanosensitive function: from biological to biomimetic channels.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.