ArticleThe Journal of cell biology2011
Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.
Article in The Journal of cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex.eLife · 2017Article
- Analysis of cell cycle parameters during the transition from unhindered growth to ribosomal and translational stress conditions.PloS one · 2017Article
- A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.The Journal of cell biology · 2016Article
- Electron Microscopy: From 2D to 3D Images with Special Reference to Muscle.European journal of translational myology · 2015Review
- Hooking She3p onto She2p for myosin-mediated cytoplasmic mRNA transport.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.RNA biology · 2015Article
- The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.The Journal of cell biology · 2014Article
- Structural insights into the assembly of a monomeric class V myosin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structure of a myosin•adaptor complex and pairing by cargo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.RNA biology · 2014Review
- A single molecule approach to mRNA transport by a class V myosin.RNA biology · 2014Article
- In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation.The Journal of cell biology · 2013Article
- Single-molecule reconstitution of mRNA transport by a class V myosin.Nature structural & molecular biology · 2013Article
- Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motors.Developmental cell · 2012Review
- Tropomyosin is essential for processive movement of a class V myosin from budding yeast.Current biology : CB · 2012Article
- Principles of mRNA transport in yeast.Cellular and molecular life sciences : CMLS · 2012Review
- Assembly of mRNA-protein complexes for directional mRNA transport in eukaryotes--an overview.Current protein & peptide science · 2012Review
- Plant-Specific Myosin XI, a Molecular Perspective.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Myo4p, one of two class V myosins in budding yeast, continuously transports messenger RNA (mRNA) cargo in the cell but is nonprocessive when characterized in vitro. The adapter protein She3p tightly binds to the Myo4p rod, forming a single-headed motor complex. In this paper, we show that two Myo4p-She3p motors are recruited by the tetrameric mRNA-binding protein She2p to form a processive double-headed complex. The binding site for She3p was mapped to a single α helix that protrudes at right angles from She2p. Processive runs of several micrometers on yeast actin-tropomyosin filaments were observed only in the presence of She2p, and, thus, motor activity is regulated by cargo binding. While moving processively, each head steps ~72 nm in a hand-over-hand motion. Coupling two high-duty cycle monomeric motors via a common cargo-binding adapter protein creates a complex with transport properties comparable with a single dimeric processive motor such as vertebrate myosin Va.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.