ArticleJournal of biophotonics2012
Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy.
Article in Journal of biophotonics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Prestin amplifies cardiac motor functions.Cell reports · 2021Article
- An intrinsic, label-free signal for identifying stem cell-derived cardiomyocyte subtype.Stem cells (Dayton, Ohio) · 2020Article
- Optical second harmonic generation microscopy: application to the sensitive detection of cell membrane damage.Biophysical reviews · 2019Review
- Discovering Macrophage Functions UsingFrontiers in immunology · 2018Review
- Generation and Assessment of Functional Biomaterial Scaffolds for Applications in Cardiovascular Tissue Engineering and Regenerative Medicine.Advanced healthcare materials · 2015Review
- Non-linear optical flow cytometry using a scanned, Bessel beam light-sheet.Scientific reports · 2015Article
- Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2014Article
- Calcium signalling of human pluripotent stem cell-derived cardiomyocytes.The Journal of physiology · 2013Review
- Concise review: maturation phases of human pluripotent stem cell-derived cardiomyocytes.Stem cells (Dayton, Ohio) · 2013Review
- Embryonic template-based generation and purification of pluripotent stem cell-derived cardiomyocytes for heart repair.Journal of cardiovascular translational research · 2012Review
- Engineered human pluripotent stem cell-derived cardiac cells and tissues for electrophysiological studies.Drug discovery today. Disease models · 2012Article
- Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency.PloS one · 2012Article
- Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells.Progress in biophysics and molecular biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC-CM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSC-CMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the non-invasive purification of PSC-CMs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.