ArticleJournal of the American Society for Mass Spectrometry2012
Detection and characterization of low abundance glycopeptides via higher-energy C-trap dissociation and orbitrap mass analysis.
Article in Journal of the American Society for Mass Spectrometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
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- ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm.Molecular and cellular biology · 2024Article
- Enrichment and fragmentation approaches for enhanced detection and characterization of endogenous glycosylated neuropeptides.Proteomics · 2023Article
- Boost-DiLeu: Enhanced IsobaricAnalytical chemistry · 2022Article
- Embedding of HIV Egress within Cortical F-Actin.Pathogens (Basel, Switzerland) · 2022Article
- Developing mass spectrometry for the quantitative analysis of neuropeptides.Expert review of proteomics · 2021Review
- Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.Molecular & cellular proteomics : MCP · 2021Review
- Analytical Guidelines for co-fractionation Mass Spectrometry Obtained through Global Profiling of Gold StandardMolecular & cellular proteomics : MCP · 2020Article
- Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally.The Journal of biological chemistry · 2020Article
- Expanding our understanding of the role of microbial glycoproteomes through high-throughput mass spectrometry approaches.Glycoconjugate journal · 2019Article
- Non-canonical ubiquitination of the cholesterol-regulated degron of squalene monooxygenase.The Journal of biological chemistry · 2019Article
- Article
- Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)-Enabled Intact Glycopeptide/Glycoproteome Characterization.Journal of the American Society for Mass Spectrometry · 2017Article
- Site-specific glycosylation of the Newcastle disease virus haemagglutinin-neuraminidase.Glycoconjugate journal · 2017Article
- A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.PLoS genetics · 2017Article
- Characterization of Glycan Structures of Chondroitin Sulfate-Glycopeptides Facilitated by Sodium Ion-Pairing and Positive Mode LC-MS/MS.Journal of the American Society for Mass Spectrometry · 2017Article
- Improved Quantitative Plant Proteomics via the Combination of Targeted and Untargeted Data Acquisition.Frontiers in plant science · 2017Article
- Classification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides.Scientific reports · 2016Article
- Large Scale Mass Spectrometry-based Identifications of Enzyme-mediated Protein Methylation Are Subject to High False Discovery Rates.Molecular & cellular proteomics : MCP · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Broad-scale mass spectrometric analyses of glycopeptides are constrained by the considerable complexity inherent to glycoproteomics, and techniques are still being actively developed to address the associated analytical difficulties. Here we apply Orbitrap mass analysis and higher-energy C-trap dissociation (HCD) to facilitate detailed insights into the compositions and heterogeneity of complex mixtures of low abundance glycopeptides. By generating diagnostic oxonium product ions at mass measurement errors of <5 ppm, highly selective glycopeptide precursor ion detections are made at sub-fmol limits of detection: analyses of proteolytic digests of a hen egg glycoprotein mixture detect 88 previously uncharacterized glycopeptides from 666 precursor ions selected for MS/MS, with only one false positive due to co-fragmentation of a non-glycosylated peptide with a glycopeptide. We also demonstrate that by (1) identifying multiple series of glycoforms using high mass accuracy single stage MS spectra, and (2) performing product ion scans at optimized HCD collision energies, the identification of peptide + N-acetylhexosamine (HexNAc) ions (Y1 ions) can be readily achieved at <5 ppm mass measurement errors. These data allow base peptide sequences and glycan compositional information to be attained with high confidence, even for glycopeptides that produce weak precursor ion signals and/or low quality MS/MS spectra. The glycopeptides characterized from low fmol abundances using these methods allow two previously unreported glycosylation sites on the Gallus gallus protein ovoglycoprotein (amino acids 82 and 90) to be confirmed; considerable glycan heterogeneities at amino acid 90 of ovoglycoprotein, and amino acids 34 and 77 of Gallus gallus ovomucoid are also revealed.
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