ArticleTransfusion2012
DARC alleles and Duffy phenotypes in African Americans.
Article in Transfusion, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Impact ofCurrent research in parasitology & vector-borne diseases · 2026Article
- CD59 gene: 143 haplotypes of 22,718 nucleotides length by computational phasing in 113 individuals from different ethnicities.Transfusion · 2024Article
- Potential hidden Plasmodium vivax malaria reservoirs from low parasitemia Duffy-negative Ethiopians: Molecular evidence.PLoS neglected tropical diseases · 2023Article
- The highest Duffy (Health science reports · 2023Article
- PopHumanVar: an interactive application for the functional characterization and prioritization of adaptive genomic variants in humans.Nucleic acids research · 2022Article
- Cataloguing experimentally confirmed 80.7 kb-long ACKR1 haplotypes from the 1000 Genomes Project database.BMC bioinformatics · 2021Article
- Defining Blood Group Gene Reference Alleles by Long-Read Sequencing: Proof of Concept in theTransfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Article
- An outcome-based review of an accredited Specialist in Blood Banking (SBB) program: 25 years and counting.Immunohematology · 2020Review
- The phylogeny of 48 alleles, experimentally verified at 21 kb, and its application to clinical allele detection.Journal of translational medicine · 2019Article
- Long-range haplotype analysis of the malaria parasite receptor geneHuman genome variation · 2018Article
- Molecular basis of the Duffy blood group system.Blood transfusion = Trasfusione del sangue · 2018Review
- Molecular typing for blood group antigens within 40 min by direct polymerase chain reaction from plasma or serum.British journal of haematology · 2017Article
- Article
- Molecular immunohaematology round table discussions at the AABB Annual Meeting, Denver 2013.Blood transfusion = Trasfusione del sangue · 2015Article
- Article
- The Lombardy Rare Donor Programme.Blood transfusion = Trasfusione del sangue · 2014Article
- Analysis for genotyping Duffy blood group in inhabitants of Sudan, the fourth cataract of the Nile.Malaria journal · 2012Article
- Erythrocyte membrane antigen frequencies in patients with Type II congenital smell loss.American journal of otolaryngologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe DARC (Duffy blood group, chemokine receptor) gene encodes for a transmembrane glycoprotein that functions as a chemokine transporter, is a receptor for Plasmodium vivax and P. knowlesi, and expresses the Duffy blood group antigens (Fy). The Fy(a-b-) phenotype found in people of African descent is typically associated with a -67t>c mutation in the 5'-untranslated region (UTR), which prevents red blood cells being invaded by P. vivax and P. knowlesi. The aim of this study was to establish DARC allele frequencies in an African American blood donor cohort, determine a phylogenetic tree for DARC, and compare human and Neandertal DARC genes. STUDY DESIGN AND
methodsThe DARC nucleotide sequence of 54 African American blood donors was determined from genomic DNA. Heterozygous substitutions were resolved by sequencing of haplotype-specific amplifications. A phylogenetic tree for DARC was established using the neighbor-joining method with Pan troglodytes as root.
resultsA total of 108 haplotypes of the DARC gene could be unambiguously determined from nucleotide position -300 in the 5' UTR to +300 in the 3' UTR. Eleven different alleles were found, including the clinically relevant FY*A, FY*B, FY*B-67C, FY*B298A, and FY*X alleles. All phenotype predictions based on genotypes matched the serologically determined phenotypes exactly: 52% Fy(a-b-), 28% Fy(a-b+), and 20% Fy(a+b-).
conclusionsThe nucleotide sequencing approach using one amplicon is a practical genotyping method for DARC and allows the determination of haplotypes even in heterozygous constellations. We developed a phylogenetic tree for DARC alleles and postulated a distinct FY*B allele as ancestral for the extant DARC alleles in humans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.