ArticleAmerican journal of physiology. Heart and circulatory physiology2012
Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance.
Article in American journal of physiology. Heart and circulatory physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 64 citations in OpenAlex.
- The influence of intercalated disk nanostructure on local ionic currents and cardiac conduction.Biophysical journal · 2026Article
- Determinants of electrical propagation and propagation block in Arrhythmogenic Cardiomyopathy.Journal of molecular and cellular cardiology · 2024Article
- Spatiotemporal cell junction assembly in human iPSC-CM models of arrhythmogenic cardiomyopathy.Stem cell reports · 2023Article
- Sodium channel subpopulations with distinct biophysical properties and subcellular localization enhance cardiac conduction.The Journal of general physiology · 2023Article
- Ephaptic Coupling as a Resolution to the Paradox of Action Potential Wave Speed and Discordant Alternans Spatial Scales in the Heart.Physical review letters · 2023Article
- Role of ephaptic coupling in discordant alternans domain sizes and action potential propagation in the heart.Physical review. E · 2023Article
- Simultaneous Widefield Voltage and Dye-Free Optical Mapping Quantifies Electromechanical Waves in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.ACS photonics · 2023Article
- A myocardial infarct border-zone-on-a-chip demonstrates distinct regulation of cardiac tissue function by an oxygen gradient.Science advances · 2022Article
- Confocal microscopy-based estimation of intracellular conductivities in myocardium for modeling of the normal and infarcted heart.Computers in biology and medicine · 2022Article
- Cellular and Engineered Organoids for Cardiovascular Models.Circulation research · 2022Review
- Serine-threonine protein phosphatase regulation of Cx43 dephosphorylation in arrhythmogenic disorders.Cellular signalling · 2021Review
- Intercalated disk nanoscale structure regulates cardiac conduction.The Journal of general physiology · 2021Article
- Characterization of Gelatin Hydrogels Cross-Linked with Microbial Transglutaminase as Engineered Skeletal Muscle Substrates.Bioengineering (Basel, Switzerland) · 2021Article
- Cellular Size, Gap Junctions, and Sodium Channel Properties Govern Developmental Changes in Cardiac Conduction.Frontiers in physiology · 2021Article
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
- Proarrhythmic Electrical Remodeling by Noncardiomyocytes at Interfaces With Cardiomyocytes Under Oxidative Stress.Frontiers in physiology · 2020Article
- Large scale and integrated platform for digital mass culture of anchorage dependent cells.Nature communications · 2019Article
- Quantifying the effects of engineered nanomaterials on endothelial cell architecture and vascular barrier integrity using a cell pair model.Nanoscale · 2019Article
- Engineering cardiac microphysiological systems to model pathological extracellular matrix remodeling.American journal of physiology. Heart and circulatory physiology · 2018Review
- Microenvironmental Modulation of Calcium Wave Propagation Velocity in Engineered Cardiac Tissues.Cellular and molecular bioengineering · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Gap junctions are composed of connexin (Cx) proteins, which mediate intercellular communication. Cx43 is the dominant Cx in ventricular myocardium, and Cx45 is present in trace amounts. Cx43 immunosignal has been associated with cell-to-cell coupling and electrical propagation, but no studies have directly correlated Cx43 immunosignal to electrical cell-to-cell conductance, g(j), in ventricular cardiomyocyte pairs. To assess the correlation between Cx43 immunosignal and g(j), we developed a method to determine both parameters from the same cell pair. Neonatal rat ventricular cardiomyocytes were seeded on micropatterned islands of fibronectin. This allowed formation of cell pairs with reproducible shapes and facilitated tracking of cell pair locations. Moreover, cell spreading was limited by the fibronectin pattern, which allowed us to increase cell height by reducing the surface area of the pattern. Whole cell dual voltage clamp was used to record g(j) of cell pairs after 3-5 days in culture. Fixation of cell pairs before removal of patch electrodes enabled preservation of cell morphology and offline identification of patched pairs. Subsequently, pairs were immunostained, and the volume of junctional Cx43 was quantified using confocal microscopy, image deconvolution, and three-dimensional reconstruction. Our results show a linear correlation between g(j) and Cx43 immunosignal within a range of 8-50 nS.
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Registered trials
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