ArticleApplied and environmental microbiology2012
Distinct transcriptional profiles and phenotypes exhibited by Escherichia coli O157:H7 isolates related to the 2006 spinach-associated outbreak.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Phenotypic and genomic comparison of three human outbreak and one cattle-associated Shiga toxin-producingMicrobiology spectrum · 2024Article
- Survival ofFoods (Basel, Switzerland) · 2021Article
- Metabolic Traits of Bovine Shiga Toxin-ProducingToxins · 2020Article
- Food-to-Humans Bacterial Transmission.Microbiology spectrum · 2020Review
- Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7.BMC genomics · 2019Article
- Assembly and Characterization of a Pathogen Strain Collection for Produce Safety Applications: Pre-growth Conditions Have a Larger Effect on Peroxyacetic Acid Tolerance Than Strain Diversity.Frontiers in microbiology · 2019Article
- Interactions of Salmonella enterica Serovar Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot.Applied and environmental microbiology · 2018Article
- A network of regulators promotes dehydration tolerance in Escherichia coli.Environmental microbiology · 2018Article
- An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits.Applied and environmental microbiology · 2016Article
- Expression of Curli byFrontiers in cellular and infection microbiology · 2016Article
- The Polymorphic Aggregative Phenotype of Shiga Toxin-Producing Escherichia coli O111 Depends on RpoS and Curli.Applied and environmental microbiology · 2015Article
- Escherichia coli survival in, and release from, white-tailed deer feces.Applied and environmental microbiology · 2015Article
- Fitness of Outbreak and Environmental Strains of Escherichia coli O157:H7 in Aerosolizable Soil and Association of Clonal Variation in Stress Gene Regulation.Pathogens (Basel, Switzerland) · 2014Article
- Enteric pathogen-plant interactions: molecular connections leading to colonization and growth and implications for food safety.Microbes and environments · 2014Review
- Complete Genome Sequence of Escherichia coli O157:H7 Bacteriophage phiJLA23 Isolated in Mexico.Genome announcements · 2013Article
- Article
- RcsB contributes to the distinct stress fitness among Escherichia coli O157:H7 curli variants of the 1993 hamburger-associated outbreak strains.Applied and environmental microbiology · 2012Article
- Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7.Applied and environmental microbiology · 2012Article
- Article
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6 authors.
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Abstract
In 2006, a large outbreak of Escherichia coli O157:H7 was linked to the consumption of ready-to-eat bagged baby spinach in the United States. The likely sources of preharvest spinach contamination were soil and water that became contaminated via cattle or feral pigs in the proximity of the spinach fields. In this study, we compared the transcriptional profiles of 12 E. coli O157:H7 isolates that possess the same two-enzyme pulsed-field gel electrophoresis (PFGE) profile and are related temporally or geographically to the above outbreak. These E. coli O157:H7 isolates included three clinical isolates, five isolates from separate bags of spinach, and single isolates from pasture soil, river water, cow feces, and a feral pig. The three clinical isolates and two spinach bag isolates grown in cultures to stationary phase showed decreased expression of many σ(S)-regulated genes, including gadA, osmE, osmY, and katE, compared with the soil, water, cow, feral pig, and the other three spinach bag isolates. The decreased expression of these σ(S)-regulated genes was correlated with the decreased resistance of the isolates to acid stress, osmotic stress, and oxidative stress but increases in scavenging ability. We also observed that intraisolate variability was much more pronounced among the clinical and spinach isolates than among the environmental isolates. Together, the transcriptional and phenotypic differences of the spinach outbreak isolates of E. coli O157:H7 support the hypothesis that some variants within the spinach bag retained characteristics of the preharvest isolates, whereas other variants with altered gene expression and phenotypes infected the human host.
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