ArticleProteins2012
A temperature-dependent conformational change of NADH oxidase from Thermus thermophilus HB8.
Article in Proteins, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed.
- Functional Characterization and Structural Analysis of NADH Oxidase Mutants fromMicroorganisms · 2019Article
- Article
- Conformational plasticity surrounding the active site of NADH oxidase from Thermus thermophilus.Protein science : a publication of the Protein Society · 2015Article
- Molecular dynamics simulations of the Nip7 proteins from the marine deep- and shallow-water Pyrococcus species.BMC structural biology · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Using molecular dynamics simulations and steady-state fluorescence spectroscopy, we have identified a conformational change in the active site of a thermophilic flavoenzyme, NADH oxidase from Thermus thermophilus HB8 (NOX). The enzyme's far-UV circular dichroism spectrum, intrinsic tryptophan fluorescence, and apparent molecular weight measured by dynamic light scattering varied little between 25 and 75°C. However, the fluorescence of the tightly bound FAD cofactor increased approximately fourfold over this temperature range. This effect appears not to be due to aggregation, unfolding, cofactor dissociation, or changes in quaternary structure. We therefore attribute the change in flavin fluorescence to a temperature-dependent conformational change involving the NOX active site. Molecular dynamics simulations and the effects of mutating aromatic residues near the flavin suggest that the change in fluorescence results from a decrease in quenching by electron transfer from tyrosine 137 to the flavin.
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Registered trials
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