ArticleJournal of bacteriology2012
Deletion of σ(54) (rpoN) alters the rate of autolysis and biofilm formation in Enterococcus faecalis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Virulence-linked adhesin drives mutualist colonization of the bee gut via biofilm formation.bioRxiv : the preprint server for biology · 2024Article
- Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation.Biotechnology letters · 2021Article
- Article
- What's a Biofilm?-How the Choice of the Biofilm Model Impacts the Protein Inventory ofFrontiers in microbiology · 2021Article
- Genome-Scale Mapping Reveals Complex Regulatory Activities of RpoN in Yersinia pseudotuberculosis.mSystems · 2020Article
- Activity of Antimicrobial Peptides and Ciprofloxacin againstMolecules (Basel, Switzerland) · 2020Article
- Single-Nucleotide RNA Maps for the Two Major Nosocomial PathogensFrontiers in cellular and infection microbiology · 2020Article
- Intestinal Bile Acids Induce a Morphotype Switch in Vancomycin-Resistant Enterococcus that Facilitates Intestinal Colonization.Cell host & microbe · 2019Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Interplay between Antibiotic Efficacy and Drug-Induced Lysis Underlies Enhanced Biofilm Formation at Subinhibitory Drug Concentrations.Antimicrobial agents and chemotherapy · 2018Article
- Effect of DNase Treatment on Adhesion and Early Biofilm Formation ofEuropean endodontic journal · 2018Article
- Genome-wide screen for genes involved in eDNA release during biofilm formation byProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Regulation ofEcoSal Plus · 2017Review
- D-Ribose Interferes with Quorum Sensing to Inhibit Biofilm Formation ofFrontiers in microbiology · 2017Article
- Effect of RpoN, RpoS and LuxS Pathways on the Biofilm Formation and Antibiotic Sensitivity ofEuropean journal of microbiology & immunology · 2016Article
- Control of Clostridium difficile Physiopathology in Response to Cysteine Availability.Infection and immunity · 2016Article
- Proteomics dedicated to biofilmology: What have we learned from a decade of research?Medical microbiology and immunology · 2016Review
- Pheromone killing of multidrug-resistant Enterococcus faecalis V583 by native commensal strains.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Whole-genome mapping of 5' RNA ends in bacteria by tagged sequencing: a comprehensive view in Enterococcus faecalis.RNA (New York, N.Y.) · 2015Article
- Genome-wide identification of genes necessary for biofilm formation by nosocomial pathogen Stenotrophomonas maltophilia reveals that orphan response regulator FsnR is a critical modulator.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transcription initiation is a critical step in bacterial gene regulation and is often controlled by transcription regulators. The alternate sigma factor (σ(54)) is one such regulator that facilitates activator-dependent transcription initiation and thus modulates the expression of a variety of genes involved in metabolism and pathogenesis in bacteria. This study describes the role of σ(54) in the nosocomial pathogen Enterococcus faecalis. Biofilm formation is one of the important pathogenic mechanisms of E. faecalis, as it elevates the organism's potential to cause surgical site and urinary tract infections. Lysis of bacterial cells within the population contributes to biofilm formation by providing extracellular DNA (eDNA) as a key component of the biofilm matrix. Deletion of rpoN rendered E. faecalis resistant to autolysis, which in turn impaired eDNA release. Despite the significant reduction in eDNA levels compared to the parental strain, the rpoN mutant formed more robust biofilms as observed using laser scanning confocal microscopy and Comstat analysis, indicating and emphasizing the presence of other matrix components. Initial adherence to a polystyrene surface was also enhanced in the mutant. Proteinase K treatment at early stages of biofilm development significantly reduced the accumulation of biofilm by the rpoN mutant. In conclusion, our data indicate that other factors in addition to eDNA might contribute to the overall composition of the enterococcal biofilm and that the regulatory role of σ(54) governs the nature and composition of the biofilm matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.