ArticleMolecular biotechnology2012
Effectiveness of AFLPs and retrotransposon-based markers for the identification of Portuguese grapevine cultivars and clones.
Article in Molecular biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Molecular Tools for Adapting Viticulture to Climate Change.Frontiers in plant science · 2021Review
- Assessment of genetic diversity of cultivated and wild Iranian grape germplasm using retrotransposon-microsatellite amplified polymorphism (REMAP) markers and pomological traits.Molecular biology reports · 2020Article
- Genetic analysis of the grapevine genotypes of the Russian Vitis ampelographic collection using iPBS markers.Genetica · 2019Article
- Use of SSR and retrotransposon-based markers to interpret the population structure of native grapevines from southern Italy.Molecular biotechnology · 2014Article
- A simple and efficient method to isolate LTR sequences of plant retrotransposon.BioMed research international · 2014Article
- Stable MSAP markers for the distinction of Vitis vinifera cv Pinot noir clones.Molecular biotechnology · 2013Article
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Authors and funding
7 authors.
Funding
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Abstract
Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.
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