ArticleMycopathologia2012
Identification of novel hybrids between Cryptococcus neoformans var. grubii VNI and Cryptococcus gattii VGII.
Article in Mycopathologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 53 citations in OpenAlex.
- Hybrids unleashed: exploring the emergence and genomic insights of pathogenic yeast hybrids.Current opinion in microbiology · 2024Review
- Horse: a potential source of Cryptococcus neoformans and Cryptococcus gattii in Egypt.BMC veterinary research · 2022Article
- The need for environmental surveillance to understand the ecology, epidemiology and impact of Cryptococcus infection in Africa.FEMS microbiology ecology · 2021Review
- Population genomic analysis of Cryptococcus Brazilian isolates reveals an African type subclade distribution.G3 (Bethesda, Md.) · 2021Article
- What Are the Best Parents for Hybrid Progeny? An Investigation into the Human Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- Molecular Markers Reveal Epidemiological Patterns and Evolutionary Histories of the Human PathogenicFrontiers in cellular and infection microbiology · 2021Review
- Fungal evolution: cellular, genomic and metabolic complexity.Biological reviews of the Cambridge Philosophical Society · 2020Article
- Review
- Pathogen and host genetics underpinning cryptococcal disease.Advances in genetics · 2020Review
- Unravelling species boundaries in thePersoonia · 2018Article
- The effects of environmental and genetic factors on the germination of basidiospores in the Cryptococcus gattii species complex.Scientific reports · 2018Article
- Molecular Characterization and Antifungal Susceptibility Testing of Sequentially Obtained Clinical Cryptococcus deneoformans and Cryptococcus neoformans Isolates from Ljubljana, Slovenia.Mycopathologia · 2018Article
- Estimating the Intra-taxa Diversity, Population Genetic Structure, and Evolutionary Pathways ofFrontiers in genetics · 2018Article
- Hybridization and emergence of virulence in opportunistic human yeast pathogens.Yeast (Chichester, England) · 2018Article
- The status of cryptococcosis in Latin America.Memorias do Instituto Oswaldo Cruz · 2018Review
- MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil.PLoS neglected tropical diseases · 2017Article
- First case of mixed infection withJMM case reports · 2016Article
- Isolation, Identification and Molecular Typing of Cryptococcus neoformans from Pigeon Droppings and Other Environmental Sources in Tripoli, Libya.Mycopathologia · 2016Article
- Article
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Authors and funding
9 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococcus neoformans and Cryptococcus gattii are pathogenic yeasts causing meningoencephalitis in immunocompromised and immunocompetent hosts. The fungus is typically haploid, and sexual reproduction occurs normally between individuals with opposite mating types, α and a. C. neoformans var. grubii (serotype A) is comprised of molecular types VNI, VNII, and VNB, and C. neoformans var. neoformans (serotype D) contains the molecular type VNIV. Additionally, diploid or aneuploid AD hybrids (VNIII) have been reported. C. gattii contains the molecular types VGI, VGII, VGIII, and VGIV, which encompass both serotypes B and C. To identify possible hybrid strains, URA5-RFLP analysis was performed on 350 globally obtained clinical, environmental, and veterinary isolates. Four clinical isolates from cerebrospinal fluid showed combination patterns of C. neoformans var. grubii and C. gattii: Brazil (n = 2), Colombia (n = 1), and India (n = 1). These strains were monokaryotic and diploid or aneuploid. M13 PCR fingerprinting showed that they contained fragments of both proposed parental groups. Luminex IGS genotyping identified these isolates as hybrids with two different molecular type combinations: three VNI/VGII and one VNI/VGI. Blue color development on CGB agar was delayed in three isolates and absent in one. C. gattii-specific PCR confirmed the presence of C. gattii in the hybrids. CAP59 allele-specific PCR revealed that all the hybrids contained both serotype A and B alleles. Determination of mating-type allelic patterns by PCR revealed that the isolates were αA aB. This is the first study discovering novel natural hybrids between C. neoformans molecular type VNI and C. gattii molecular type VGII.
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