ArticlePlant physiology2012
Overlapping photoprotective function of vitamin E and carotenoids in Chlamydomonas.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Microalgae as a source of α-tocopherol: phylogenetic and technological factors.World journal of microbiology & biotechnology · 2026Review
- Antioxidant Compounds in Microalgae and Cyanobacteria: A Sustainable Source of Bioactive Molecules.Marine drugs · 2026Review
- Knock Down ofPlants (Basel, Switzerland) · 2025Article
- Effects of high light exposure and heterologous expression of β-carotene ketolase on the metabolism of carotenoids inFrontiers in bioengineering and biotechnology · 2025Article
- Exploring the Potential of Carotenoids in Obesity Management: A Comprehensive Overview.Recent advances in food, nutrition & agriculture · 2025Review
- Cold-Induced Physiological and Biochemical Alternations and Proteomic Insight into the Response ofInternational journal of molecular sciences · 2022Article
- Article
- Chlamydomonas as a model for reactive oxygen species signaling and thiol redox regulation in the green lineage.Plant physiology · 2021Review
- Discovery of photosynthesis genes through whole-genome sequencing of acetate-requiring mutants of Chlamydomonas reinhardtii.PLoS genetics · 2021Article
- Mechanisms underlying mercury detoxification in soil-plant systems after selenium application: a review.Environmental science and pollution research international · 2021Review
- Antioxidant and Signaling Role of Plastid-Derived Isoprenoid Quinones and Chromanols.International journal of molecular sciences · 2021Review
- Genetically Programmed Changes in Photosynthetic Cofactor Metabolism in Copper-deficient Chlamydomonas.The Journal of biological chemistry · 2016Article
- Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii.Journal of experimental botany · 2015Article
- Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants.PloS one · 2015Article
- A dual strategy to cope with high light in Chlamydomonas reinhardtii.The Plant cell · 2013Article
- Desiccation induces accumulations of antheraxanthin and zeaxanthin in intertidal macro-alga Ulva pertusa (Chlorophyta).PloS one · 2013Article
- The MORPH algorithm: ranking candidate genes for membership in Arabidopsis and tomato pathways.The Plant cell · 2012Article
- Article
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Authors and funding
3 authors.
Funding
Abstract
Tocopherols (vitamin E) and carotenoids are the two most abundant groups of lipid-soluble antioxidants in the chloroplast. Carotenoids are well known for their roles in protecting against photooxidative stress, whereas the photoprotective functions of tocopherols have only recently been examined experimentally. In addition, little is known about the functional overlap of carotenoids and tocopherols in vivo. To investigate this possible overlap, Chlamydomonas reinhardtii strains were engineered to overproduce tocopherols by chloroplast transformation with non-codon-optimized and codon-optimized versions of the homogentisate phytyltransferase vitamin E2 (VTE2) from Synechocystis and by nuclear transformation with VTE2 from C. reinhardtii, which resulted in 1.6-fold, 5-fold to 10-fold, and more than 10-fold increases in total tocopherol content, respectively. To test if tocopherol overproduction can compensate for carotenoid deficiency in terms of antioxidant function, the nuclear VTE2 gene from C. reinhardtii was overexpressed in the npq1 lor1 double mutant, which lacks zeaxanthin and lutein. Following transfer to high light, the npq1 lor1 strains that overaccumulated tocopherols showed increased resistance for up to 2 d and higher efficiency of photosystem II, and they were also much more resistant to other oxidative stresses. These results suggest an overlapping functions of tocopherols and carotenoids in protection against photooxidative stress.
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