ArticleApplied microbiology and biotechnology2012
A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background.
Article in Applied microbiology and biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 83 citations in OpenAlex.
- A ZnCommunications biology · 2025Article
- Improvement of the capacity to hydrolyze corncob residues through deciphering the lignocellulolytic enzyme system of a hyper-cellulolytic mutant strain Trichoderma reesei CU7-4.Biotechnology for biofuels and bioproducts · 2025Article
- Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures.Journal of biological engineering · 2025Article
- Light controls gene functions through alternative splicing in fungi.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- N-acetylglucosamine sensing in the filamentous soil fungus Trichoderma reesei.The FEBS journal · 2025Article
- Catalytic Efficiency Improvement in Cellobiohydrolase I by Cross-Species Domain Exchange Engineering.International journal of molecular sciences · 2025Article
- Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum.Current microbiology · 2025Article
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- Promoters and Synthetic Promoters in Trichoderma reesei.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Enhanced Expression of Alcohol Dehydrogenase I inMicroorganisms · 2023Article
- Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production inJournal of fungi (Basel, Switzerland) · 2023Article
- The bZIP transcription factor ATF1 regulates blue light and oxidative stress responses inmLife · 2023Article
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.Bioresources and bioprocessing · 2022Article
- Heterologous Expression of Secondary Metabolite Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- Construction of a novel filamentous fungal protein expression system based on redesigning of regulatory elements.Applied microbiology and biotechnology · 2022Article
- The pleiotropic functions of intracellular hydrophobins in aerial hyphae and fungal spores.PLoS genetics · 2021Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Recent demands for the production of biofuels from lignocellulose led to an increased interest in engineered cellulases from Trichoderma reesei or other fungal sources. While the methods to generate such mutant cellulases on DNA level are straightforward, there is often a bottleneck in their production since a correct posttranslational processing of these enzymes is needed to obtain highly active enzymes. Their production and subsequent enzymatic analysis in the homologous host T. reesei is, however, often disturbed by the concomitant production of other endogenous cellulases. As a useful alternative, we tested the production of cellulases in T. reesei in a genetic background where cellulase formation has been impaired by deletion of the major cellulase transcriptional activator gene xyr1. Three cellulase genes (cel7a, cel7b, and cel12a) were expressed under the promoter regions of the two highly expressed genes tef1 (encoding translation elongation factor 1-alpha) or cdna1 (encoding the hypothetical protein Trire2:110879). When cultivated on D: -glucose as carbon source, the Δxyr1 strain secreted all three cellulases into the medium. Related to the introduced gene copy number, the cdna1 promoter appeared to be superior to the tef1 promoter. No signs of proteolysis were detected, and the individual cellulases could be assayed over a background essentially free of other cellulases. Hence this system can be used as a vehicle for rapid and high-throughput testing of cellulase muteins in a homologous background.
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