ArticleJournal of plant research2012
Involvement of the putative Ca²⁺-permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca²⁺ uptake, Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotiana tabacum) BY-2 cells.
Article in Journal of plant research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 66 citations in OpenAlex.
- Influence of sound vibrations on plant holobionts: physiological pathways linking root function and rhizospheric microbial interactions.Plant signaling & behavior · 2026Review
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- The viscoelastic properties of Nicotiana tabacum BY-2 suspension cell lines adapted to high osmolarity.BMC plant biology · 2025Article
- Salinity survival: molecular mechanisms and adaptive strategies in plants.Frontiers in plant science · 2025Review
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- Sound waves alter the viability of tobacco cells via changes in cytosolic calcium, membrane integrity, and cell wall composition.PloS one · 2024Article
- Calcium Signaling and the Response to Heat Shock in Crop Plants.International journal of molecular sciences · 2023Review
- Review
- Origin, evolution and diversification of plant mechanosensitive channel of small conductance-like (MSL) proteins.BMC plant biology · 2023Article
- Calcium signaling-mediated transcriptional reprogramming during abiotic stress response in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Review
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Molecular Mechanisms of Plant Responses to Salt Stress.Frontiers in plant science · 2022Review
- Sensing Mechanisms: Calcium Signaling Mediated Abiotic Stress in Plants.Frontiers in plant science · 2022Review
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Mix and match: Patchwork domain evolution of the land plant-specific Ca2+-permeable mechanosensitive channel MCA.PloS one · 2021Article
- MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls.Plants (Basel, Switzerland) · 2020Article
- Plasma Membrane CaInternational journal of molecular sciences · 2020Article
- Hitting the Wall-Sensing and Signaling Pathways Involved in Plant Cell Wall Remodeling in Response to Abiotic Stress.Plants (Basel, Switzerland) · 2018Review
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To gain insight into the cellular functions of the mid1-complementing activity (MCA) family proteins, encoding putative Ca²⁺-permeable mechanosensitive channels, we isolated two MCA homologs of tobacco (Nicotiana tabacum) BY-2 cells, named NtMCA1 and NtMCA2. NtMCA1 and NtMCA2 partially complemented the lethality and Ca²⁺ uptake defects of yeast mutants lacking mechanosensitive Ca²⁺ channel components. Furthermore, in yeast cells overexpressing NtMCA1 and NtMCA2, the hypo-osmotic shock-induced Ca²⁺ influx was enhanced. Overexpression of NtMCA1 or NtMCA2 in BY-2 cells enhanced Ca²⁺ uptake, and significantly alleviated growth inhibition under Ca²⁺ limitation. NtMCA1-overexpressing BY-2 cells showed higher sensitivity to hypo-osmotic shock than control cells, and induced the expression of the touch-inducible gene, NtERF4. We found that both NtMCA1-GFP and NtMCA2-GFP were localized at the plasma membrane and its interface with the cell wall, Hechtian strands, and at the cell plate and perinuclear vesicles of dividing cells. NtMCA2 transcript levels fluctuated during the cell cycle and were highest at the G1 phase. These results suggest that NtMCA1 and NtMCA2 play roles in Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in BY-2 cells, by regulating the Ca²⁺ influx through the plasma membrane.
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