ArticleJournal of molecular biology2012
YhiQ is RsmJ, the methyltransferase responsible for methylation of G1516 in 16S rRNA of E. coli.
Article in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Characterization of Tellurite Toxicity toInternational journal of molecular sciences · 2025Article
- Post-Transcriptional Modifications of the Large Ribosome Subunit Assembly Intermediates inBiochemistry · 2025Article
- Unveiling metabolic integration in psyllids and their nutritional endosymbionts through comparative transcriptomics analysis.iScience · 2023Article
- The emerging role of epitranscriptome in shaping stress responses in plants.Plant cell reports · 2023Review
- Integration of transcriptomic and proteomic analyses of cold shock response in Kosmotoga olearia, a typical thermophile with an incredible minimum growth temperature at 20 °C.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- 16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis.The protein journal · 2022Review
- Review
- Comprehensive Functional Analysis ofFrontiers in genetics · 2020Article
- A diversity-oriented rhodamine library for wide-spectrum bactericidal agents with low inducible resistance against resistant pathogens.Nature communications · 2019Article
- Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization inFrontiers in microbiology · 2019Article
- Structural and evolutionary insights into ribosomal RNA methylation.Nature chemical biology · 2018Review
- Comparative RNA function analysis reveals high functional similarity between distantly related bacterial 16 S rRNAs.Scientific reports · 2017Article
- Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite.Microbial cell factories · 2015Article
- Evolutionary and sequence-based relationships in bacterial AdoMet-dependent non-coding RNA methyltransferases.BMC research notes · 2014Article
- Aminoglycoside resistance 16S rRNA methyltransferases block endogenous methylation, affect translation efficiency and fitness of the host.RNA (New York, N.Y.) · 2014Article
- Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs.RNA biology · 2013Article
- Molecular characterization of an rsmD-like rRNA methyltransferase from the Wolbachia endosymbiont of Brugia malayi and antifilarial activity of specific inhibitors of the enzyme.Antimicrobial agents and chemotherapy · 2013Article
- EcoGene 3.0.Nucleic acids research · 2013Article
- The last rRNA methyltransferase of E. coli revealed: the yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA.RNA (New York, N.Y.) · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ten methyltransferases and one pseudouridine synthase are required for complete modification of the small ribosomal subunit in Escherichia coli. Nine methyltransferases, as well as the pseudouridine synthase, are already known. Here, we identify RsmJ, the last unknown methyltransferase required for methylation of m(2)G1516 in 16S ribosomal RNA (rRNA), as the protein encoded by yhiQ. Reverse transcription primer extension analysis reveals that rRNA extracted from a yhiQ deletion strain is not methylated at G1516. Moreover, methylation is restored upon gene complementation. Also, purified recombinant YhiQ specifically methylates 30S subunits extracted from the deletion strain. The absence of the yhiQ gene leads to a cold-sensitive phenotype. Based on these data, we propose that the yhiQ gene be renamed rsmJ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.