ArticleBrain research2012
Effects of A-CREB, a dominant negative inhibitor of CREB, on the expression of c-fos and other immediate early genes in the rat SON during hyperosmotic stimulation in vivo.
Article in Brain research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Taurine improves the differentiation of neural stem cells in fetal rats with intrauterine growth restriction via activation of the PKA-CREB-BDNF signaling pathway.Metabolic brain disease · 2021Article
- TrkB signaling regulates the cold-shock protein RBM3-mediated neuroprotection.Life science alliance · 2021Article
- Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: OPPOSING ROLES FOR THE TRANSCRIPTION FACTORS CREB (cAMP RESPONSE ELEMENT BINDING) PROTEIN AND Sp1 (STIMULATORY PROTEIN-1).The Journal of biological chemistry · 2016Article
- Rasd1, a small G protein with a big role in the hypothalamic response to neuronal activation.Molecular brain · 2016Article
- Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus.Molecular brain · 2015Article
- A comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.American journal of physiology. Renal physiology · 2015Article
- A RNA-Seq Analysis of the Rat Supraoptic Nucleus Transcriptome: Effects of Salt Loading on Gene Expression.PloS one · 2015Article
- The use of protein-DNA, chromatin immunoprecipitation, and transcriptome arrays to describe transcriptional circuits in the dehydrated male rat hypothalamus.Endocrinology · 2014Article
- Transcription factor CREB3L1 regulates vasopressin gene expression in the rat hypothalamus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Introducing Genes With Significant Role in Migraine: An Interactomic Approach.Basic and clinical neuroscienceArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Intraperitoneal administration of hypertonic saline to the rat supraoptic nucleus (SON) increases the expression of several immediate early genes (IEG) and the vasopressin gene. These increases have usually been attributed to action of the cyclic-AMP Response Element Binding Protein (CREB). In this paper, we study the role of CREB in these events in vivo by delivering a potent dominant-negative form of CREB, known as A-CREB, to the rat SON through the use of an adeno-associated viral (AAV) vector. Preliminary experiments on HEK 293 cells in vitro showed that the A-CREB vector that we used completely eliminated CREB-induced c-fos expression. We stereotaxically injected this AAV-A-CREB into one SON and a control AAV into the contralateral SON of the same rat. Two weeks following these injections we injected hypertonic saline intraperitoneally into the rat. Using this paradigm, we could measure the relative effects of inhibiting CREB on the induced expression of c-fos, ngfi-a, ngfi-b, and vasopressin genes in the A-CREB AAV injected SON versus the control AAV injected SON in the same rat. We found only a small (20%) decrease of c-fos expression and a 30% decrease of ngfi-b expression in the presence of the A-CREB. There were no significant changes in expression found in the other IEGs nor in vasopressin that were produced by the A-CREB. This suggests that CREB may play only a minor role in the expression of IEGs and vasopressin in the osmotically activated SON in vivo.
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