ArticleBiochemical and biophysical research communications2011
High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells.
Article in Biochemical and biophysical research communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Hyperglycaemia-induced impairment of the autorhythmicity and gap junction activity of mouse embryonic stem cell-derived cardiomyocyte-like cells.Histochemistry and cell biology · 2023Article
- Scalable in vitro production of defined mouse erythroblasts.PloS one · 2022Article
- Insights to Heart Development and Cardiac Disease Models Using Pluripotent Stem Cell Derived 3D Organoids.Frontiers in cell and developmental biology · 2021Review
- Fabrication of Micro- and Nanopillars from Pyrolytic Carbon and Tetrahedral Amorphous Carbon.Micromachines · 2019Article
- The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models.Physiological reports · 2019Article
- An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality.Genes & development · 2018Article
- Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells.Scientific reports · 2016Article
- Forced aggregation and defined factors allow highly uniform-sized embryoid bodies and functional cardiomyocytes from human embryonic and induced pluripotent stem cells.Heart and vessels · 2014Article
- Global warming and neurodegenerative disorders: speculations on their linkage.BioImpacts : BI · 2014Article
- Functional cardiomyocytes derived from Isl1 cardiac progenitors via Bmp4 stimulation.PloS one · 2014Article
- Paramagnetic beads and magnetically mediated strain enhance cardiomyogenesis in mouse embryoid bodies.PloS one · 2014Article
- Human pluripotent stem cell-derived cardiomyocytes as research and therapeutic tools.BioMed research international · 2014Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Murine embryonic stem cell (mESC)-derived cardiomyocytes represent a promising source of cells for use in the development of models for studying early cardiac development as well as cell-based therapies in postnatal pathologies. Here, we report a highly efficient cardiac differentiation system in which high density embryoid body (EB) cultures leads to a marked increase of cardiomyocytes production from multiple mESC lines without the addition of any cardiogenic growth factors. Our results show that high density EB cultures significantly increase the yield of functional cardiomyocytes, which express typical cardiac markers, exhibit normal rhythmic Ca(2+) transients, and respond to both β-adrenergic and electric stimulations. During the differentiation period, the inhibition of bone morphogenetic protein (BMP) signaling significantly attenuates the increase of cardiac differentiation as well as the increased expression of cardiac-specific genes, NK2 transcription factor related 5 (Nkx2.5) and myosin light chain 2v (Mlc2v) by high density EB cultures. Therefore, we believe that we offer a novel and efficient means of cardiomyocyte production for practical use of mESCs in cardiac regenerative medicine.
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