Evidence map›Paper›PMID 22077724›Full record

ArticleThe New phytologist2012

An assessment of transgenomics as a tool for identifying genes involved in the evolutionary differentiation of closely related plant species.

Raul Correa, John Stanga, Bret Larget, Aaron Roznowski, Guoping Shu, Brian Dilkes, David A Baum

Open access · bronzeAbstract read
In one paragraph

Article in The New phytologist, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. A Comprehensive Review on Chickpea (International journal of molecular sciences · 2022
    Review
  3. International journal of molecular sciences · 2021
    Review
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Raul CorreaDepartment of Botany, University of Wisconsin-Madison, Madison, WI 53706, USA.
John Stanga
Bret Larget
Aaron Roznowski
Guoping Shu
Brian Dilkes
David A Baum
University of Wisconsin–Madison · USHarvard University · USPurdue University West Lafayette · US

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007133 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 1985 to 2023
$16.5M
NIGMS NIH HHS T32 GM007133
6 · The paper itself

Abstract

• Transgenomics is the process of introducing genomic clones from a donor species into a recipient species and then screening the resultant transgenic lines for phenotypes of interest. This method might allow us to find genes involved in the evolution of phenotypic differences between species as well as genes that have the potential to contribute to reproductive isolation: potential speciation genes. • More than 1100 20-kbp genomic clones from Leavenworthia alabamica were moved into Arabidopsis thaliana by transformation. After screening a single primary transformant for each line, clones associated with mutant phenotypes were tested for repeatability and co-segregation. • We found 84 clones with possible phenotypic effects, of which eight were repeatedly associated with the same phenotype. One clone, 11_11B, co-segregated with a short fruit phenotype. Further study showed that 11_11B affects seed development, with as much as one-third of the seeds aborted in some fruit. • Transgenomics is a viable strategy for discovering genes of evolutionary interest. We identify methods to reduce false positives and false negatives in the future. 11_11B can be viewed as a potential speciation gene, illustrating the value of transgenomics for studying the molecular basis of reproductive isolation.

Indexed as

Biological EvolutionPhylogenyArabidopsisBrassicaceaeChromosome SegregationCrosses, GeneticFruitGenes, PlantGenomicsHigh-Throughput Screening AssaysModels, BiologicalPhenotypeSeedsSpecies SpecificityTransformation, Genetic

Identifiers

PMID22077724
PMCPMC3253215
OpenAlexW2128776599

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.