ArticleCirculation research2012
Integration of repulsive guidance cues generates avascular zones that shape mammalian blood vessels.
Article in Circulation research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 63 citations in OpenAlex.
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- Transcription Landscape of the Early Developmental Biology in Pigs.Animals : an open access journal from MDPI · 2021Article
- The migratory pathways of the cells that form the endocardium, dorsal aortae, and head vasculature in the mouse embryo.BMC developmental biology · 2021Article
- Duplication of the Inferior Vena Cava Associated with Open Spinal Dysraphism.Clinical neuroradiology · 2021Article
- Identification of a novel heterozygous missense mutation of SEMA3E (c.1327G>A; p. Ala443Thr) in a labor induced fetus with CHARGE syndrome.Molecular genetics & genomic medicine · 2020Article
- Semaphorins in Angiogenesis and Autoimmune Diseases: Therapeutic Targets?Frontiers in immunology · 2020Review
- Class-3 Semaphorins and Their Receptors: Potent Multifunctional Modulators of Tumor Progression.International journal of molecular sciences · 2019Review
- Review
- Role of Semaphorin Signaling During Cardiovascular Development.Journal of the American Heart Association · 2018Review
- Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis Through Regulation of VEGF Signaling.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1.The Journal of cell biology · 2016Article
- The role of the semaphorins in cancer.Cell adhesion & migration · 2016Review
- Class 3 semaphorins in cardiovascular development.Cell adhesion & migration · 2016Review
- Blood vessel crosstalk during organogenesis-focus on pancreas and endothelial cells.Wiley interdisciplinary reviews. Developmental biology · 2016Review
- Notochord Cells in Intervertebral Disc Development and Degeneration.Journal of developmental biology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
rationalePositive signals, such as vascular endothelial growth factor, direct endothelial cells (ECs) to specific locations during blood vessel formation. Less is known about repulsive signal contribution to shaping vessels. Recently, "neuronal guidance cues" have been shown to influence EC behavior, particularly in directing sprouting angiogenesis by repelling ECs. However, their role during de novo blood vessel formation remains unexplored.
objectiveTo identify signals that guide and pattern the first mammalian blood vessels. METHODS AND
resultsUsing genetic mouse models, we show that blood vessels are sculpted through the generation of stereotyped avascular zones by EC-repulsive cues. We demonstrate that Semaphorin3E (Sema3E) is a key factor that shapes the paired dorsal aortae in mouse, as sema3E(-/-) embryos develop an abnormally branched aortic plexus with a markedly narrowed avascular midline. In vitro cultures and avian grafting experiments show strong repulsion of ECs by Sema3E-expressing cells. We further identify the mouse notochord as a rich source of multiple redundant neuronal guidance cues. Mouse embryos that lack notochords fail to form cohesive aortic vessels because of loss of the avascular midline, yet maintain lateral avascular zones. We demonstrate that lateral avascular zones are directly generated by the lateral plate mesoderm, a critical source of Sema3E.
conclusionsThese findings demonstrate that Sema3E-generated avascular zones are critical regulators of mammalian cardiovascular patterning and are the first to identify a repulsive role for the lateral plate mesoderm. Integration of multiple, and in some cases redundant, repulsive cues from various tissues is critical to patterning the first embryonic blood vessels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.