ArticleBiomedical optics express2011
Light assisted antibody immobilization for bio-sensing.
Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Photoinduced Immobilization on Two-Dimensional Nano Borophene Spatially Orients Capture Antibody for Highly Sensitive Biological Interactions.ACS central science · 2025Article
- Needle-Shaped Biosensors for Precision Diagnoses: From Benchtop Development to In Vitro and In Vivo Applications.Biosensors · 2024Review
- Review
- Tools to compare antibody gold nanoparticle conjugates for a small molecule immunoassay.Mikrochimica acta · 2023Article
- Experimental Study of a Quad-Band Metamaterial-Based Plasmonic Perfect Absorber as a Biosensor.Molecules (Basel, Switzerland) · 2022Article
- Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood.Nature communications · 2020Article
- Article
- Screen Printed Based Impedimetric Immunosensor for Rapid Detection ofSensors (Basel, Switzerland) · 2020Article
- Colorimetric Immunosensor by Aggregation of Photochemically Functionalized Gold Nanoparticles.ACS omega · 2018Article
- QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions.Polymers · 2017Article
- Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development.Methods (San Diego, Calif.) · 2017Review
- Article
- A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor.Journal of virological methods · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultrashort UV pulses at 258 nm with repetition rate of 10 kHz have been used to irradiate buffer solution of antibody. The tryptophan residues strongly absorb this radiation thus becoming capable to disrupt the disulfide bridges located next to them. Due to their high reactivity the opened bridges can anchor a gold plate more efficiently than other sites of the macromolecule giving rise to preferential orientations of the variable part of the antibody. UV irradiation has been applied to anchor antiIgG antibody to the electrode of a Quartz Crystal Microbalance (QCM) that lends itself as a sensor, the antibody acting as the bio-receptor. An increase of the QCM sensitivity and of the linear range has been measured when the antibody is irradiated with UV laser pulses. The photo-induced reactions leading to disulfide bridge breakage have been analyzed by means of a chemical assay that confirms our explanation. The control of disulfide bridges by UV light paves the way to important applications for sensing purpose since cysteine in combination with tryptophan can act as a hook to link refractory bio-receptors to surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.