ArticlePloS one2011
LINE-1 methylation levels in leukocyte DNA and risk of renal cell cancer.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 60 citations in OpenAlex.
- LINE-1 hypomethylation in blood and tissue samples as an epigenetic marker for cancer risk: a systematic review and meta-analysis.PloS one · 2014Pooled it
- Global DNA hypomethylation in peripheral blood leukocytes as a biomarker for cancer risk: a meta-analysis.PloS one · 2012Pooled it
- Prospective study of DNA methylation at LINE-1 and Alu in peripheral blood and the risk of prostate cancer.The Prostate · 2015Trial
- No effect of weight loss on LINE-1 methylation levels in peripheral blood leukocytes from postmenopausal overweight women.Obesity (Silver Spring, Md.) · 2014Trial
- LINE1 methylation levels associated with increased bladder cancer risk in pre-diagnostic blood DNA among US (PLCO) and European (ATBC) cohort study participants.Epigenetics · 2014Trial
- Targeting DNA methylation: molecular and in vivo evidence for quinoa and chia nanocapsules as epigenetic modulators in DMBA-treated rats.NPJ science of food · 2026Article
- Mendelian randomization analysis reveals causal relationships between circulating cell traits and renal disorders.Frontiers in medicine · 2024Article
- Article
- DNA methylation level in blood and relations to breast cancer, risk factors and environmental exposure in Greenlandic Inuit women.Basic & clinical pharmacology & toxicology · 2020Article
- Epigenome-based cancer risk prediction: rationale, opportunities and challenges.Nature reviews. Clinical oncology · 2018Review
- Single-walled and multi-walled carbon nanotubes induce sequence-specific epigenetic alterations in 16 HBE cells.Oncotarget · 2018Article
- Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals.The journal of nutrition, health & aging · 2018Article
- Article
- DNA methylation/hydroxymethylation in melanoma.Oncotarget · 2017Review
- Multiple gene-specific DNA methylation in blood leukocytes and colorectal cancer risk: a case-control study in China.Oncotarget · 2017Article
- LINE-1 hypermethylation in white blood cell DNA is associated with high-grade cervical intraepithelial neoplasia.BMC cancer · 2017Article
- Associations between Global DNA Methylation and Telomere Length in Healthy Adolescents.Scientific reports · 2017Article
- Prenatal airborne polycyclic aromatic hydrocarbon exposure, LINE1 methylation and child development in a Chinese cohort.Environment international · 2017Article
- The epigenetic landscape of renal cancer.Nature reviews. Nephrology · 2017Review
- Relationships between Global DNA Methylation in Circulating White Blood Cells and Breast Cancer Risk Factors.Journal of cancer epidemiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 8 institutions in 4 countries.
Funding
Abstract
purposeLeukocyte global DNA methylation levels are currently being considered as biomarkers of cancer susceptibility and have been associated with risk of several cancers. In this study, we aimed to examine the association between long interspersed nuclear elements (LINE-1) methylation levels, as a biomarker of global DNA methylation in blood cell DNA, and renal cell cancer risk. EXPERIMENTAL
designLINE-1 methylation of bisulfite-converted genomic DNA isolated from leukocytes was quantified by pyrosequencing measured in triplicate, and averaged across 4 CpG sites. A total of 328 RCC cases and 654 controls frequency-matched(2∶1) on age(±5years), sex and study center, from a large case-control study conducted in Central and Eastern Europe were evaluated.
resultsLINE-1 methylation levels were significantly higher in RCC cases with a median of 81.97% (interquartile range[IQR]: 80.84-83.47) compared to 81.67% (IQR: 80.35-83.03) among controls (p = 0.003, Wilcoxon). Compared to the lowest LINE-1 methylation quartile(Q1), the adjusted ORs for increasing methylation quartiles were as follows: OR(Q2) = 1.84(1.20-2.81), OR(Q3) = 1.72(1.11-2.65) and OR(Q4) = 2.06(1.34-3.17), with a p-trend = 0.004. The association was stronger among current smokers (p-trend<0.001) than former or never smokers (p-interaction = 0.03). To eliminate the possibility of selection bias among controls, the relationship between LINE-1 methylation and smoking was evaluated and confirmed in a case-only analysis, as well.
conclusionsHigher levels of LINE-1 methylation appear to be positively associated with RCC risk, particularly among current smokers. Further investigations using both post- and pre-diagnostic genomic DNA is warranted to confirm findings and will be necessary to determine whether the observed differences occur prior to, or as a result of carcinogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.