ArticlePloS one2011
Derivation, characterization, and stable transfection of induced pluripotent stem cells from Fischer344 rats.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Generation of induced pluripotent stem cells from rat fibroblasts and optimization of its differentiation into mature functional neurons.Journal of neuroscience methods · 2024Article
- Actively transcribed rDNA and distal junction (DJ) sequence are involved in association of NORs with nucleoli.Cellular and molecular life sciences : CMLS · 2023Article
- Dual Mode of Mitochondrial ROS Action during Reprogramming to Pluripotency.International journal of molecular sciences · 2022Article
- Differentiation and characterization of neurons derived from rat iPSCs.Journal of neuroscience methods · 2020Article
- Genetic tool for fate mapping of Oct4 (Pou5f1)-expressing cells and their progeny past the pluripotency stage.Stem cell research & therapy · 2019Article
- Article
- Immortalized murine fibroblast cell lines are refractory to reprogramming to pluripotent state.Oncotarget · 2018Article
- Article
- Induced Pluripotent Stem Cell-Derived Endothelial Cells Overexpressing Interleukin-8 Receptors A/B and/or C-C Chemokine Receptors 2/5 Inhibit Vascular Injury Response.Stem cells translational medicine · 2017Article
- Derivation of Transgene-Free Rat Induced Pluripotent Stem Cells Approximating the Quality of Embryonic Stem Cells.Stem cells translational medicine · 2017Article
- Cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice.Cell cycle (Georgetown, Tex.) · 2015Article
- Dual gene expression in embryoid bodies derived from human induced pluripotent stem cells using episomal vectors.Tissue engineering. Part A · 2014Article
- Inhibition of protein kinase C signaling maintains rat embryonic stem cell pluripotency.The Journal of biological chemistry · 2013Article
- Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.PloS one · 2013Article
- Article
- The Profile of Post-translational Modifications of Histone H1 in Chromatin of Mouse Embryonic Stem Cells.Acta naturaeArticle
- Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
The rat represents an important animal model that, in many respects, is superior to the mouse for dissecting behavioral, cardiovascular and other physiological pathologies relevant to humans. Derivation of induced pluripotent stem cells from rats (riPS) opens the opportunity for gene targeting in specific rat strains, as well as for the development of new protocols for the treatment of different degenerative diseases. Here, we report an improved lentivirus-based hit-and-run riPS derivation protocol that makes use of small inhibitors of MEK and GSK3. We demonstrate that the excision of proviruses does not affect either the karyotype or the differentiation ability of these cells. We show that the established riPS cells are readily amenable to genetic manipulations such as stable electroporation. Finally, we propose a genetic tool for an improvement of riPS cell quality in culture. These data may prompt iPS cell-based gene targeting in rat as well as the development of iPS cell-based therapies using disease models established in this species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.