ArticleMicrobes and environments2012
Evaluation of methods for the extraction of DNA from drinking water distribution system biofilms.
Article in Microbes and environments, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Long-term influences of pipe materials on bacterial communities of matured biofilms (> 40 years' old) in drinking water distribution systems.Fundamental research · 2026Article
- Detection and removal methods of antibiotic-resistance genes in drinking water sources: a review.Journal of water and health · 2026Review
- The impact of DNA extraction methods on species quantification and apparent community composition of in vitro oral biofilms.FEMS microbiology letters · 2026Article
- The impact of DNA extraction on the quantification ofMicrobiology spectrum · 2024Article
- A rapid, inexpensive and effective method for the efficient isolation of genomic DNA from Gram-negative bacteria.Molecular genetics and genomics : MGG · 2024Article
- Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water.Scientific reports · 2024Article
- Evaluation of DNA extraction yield from a chlorinated drinking water distribution system.PloS one · 2021Article
- Validating DNA Extraction Protocols for Bentonite Clay.mSphere · 2019Article
- Article
- Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling.Scientific reports · 2018Article
- Drinking water microbiome assembly induced by water stagnation.The ISME journal · 2018Article
- Article
- Suitability of various DNA extraction methods for a traditional Chinese paocai system.Bioengineered · 2017Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Benefits of Genomic Insights and CRISPR-Cas Signatures to Monitor Potential Pathogens across Drinking Water Production and Distribution Systems.Frontiers in microbiology · 2017Article
- Core-satellite populations and seasonality of water meter biofilms in a metropolitan drinking water distribution system.The ISME journal · 2016Article
- Comparison of Particle-Associated Bacteria from a Drinking Water Treatment Plant and Distribution Reservoirs with Different Water Sources.Scientific reports · 2016Article
- Bacterial community analysis of drinking water biofilms in southern Sweden.Microbes and environments · 2015Article
- Impact of hydraulic well restoration on native bacterial communities in drinking water wells.Microbes and environments · 2014Article
- Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing.Journal of oral microbiology · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While drinking water biofilms have been characterized in various drinking water distribution systems (DWDS), little is known about the impact of different DNA extraction methods on the subsequent analysis of microbial communities in drinking water biofilms. Since different DNA extraction methods have been shown to affect the outcome of microbial community analysis in other environments, it is necessary to select a DNA extraction method prior to the application of molecular tools to characterize the complex microbial ecology of the DWDS. This study compared the quantity and quality of DNA yields from selected DWDS bacteria with different cell wall properties using five widely used DNA extraction methods. These were further selected and evaluated for their efficiency and reproducibility of DNA extraction from DWDS samples. Terminal restriction fragment length analysis and the 454 pyrosequencing technique were used to interpret the differences in microbial community structure and composition, respectively, from extracted DNA. Such assessments serve as a concrete step towards the determination of an optimal DNA extraction method for drinking water biofilms, which can then provide a reliable comparison of the meta-analysis results obtained in different laboratories.
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