ArticleJournal of clinical microbiology2012
Simultaneous detection of six diarrhea-causing bacterial pathogens with an in-house PCR-luminex assay.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 84 citations in OpenAlex.
- Diagnostic Accuracy of Multiplex NAAT/PCR and Culture AgainstPathogens (Basel, Switzerland) · 2025Pooled it
- Systematic Review and Meta-Analyses Assessment of the Clinical Efficacy of Bismuth Subsalicylate for Prevention and Treatment of Infectious Diarrhea.Digestive diseases and sciences · 2021Pooled it
- Impact of enterovirus and other enteric pathogens on oral polio and rotavirus vaccine performance in Bangladeshi infants.Vaccine · 2016Trial
- Gastrointestinal pathogens in paediatric patients with diarrhoea during the COVID-19 pandemic in Spain: a multicentre molecular-based prospective study.European journal of pediatrics · 2026Article
- Association of coagulase-negative staphylococci with orthopedic infections detected by in-house multiplex real-time PCR.Frontiers in microbiology · 2024Article
- Prevalence of Intestinal Parasitic Infections, Genotypes, and Drug Susceptibility ofTropical medicine and infectious disease · 2023Article
- The application research of xTAG GPP multiplex PCR in the diagnosis of persistent and chronic diarrhea in children.BMC pediatrics · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Considerations for using ETEC and Shigella disease burden estimates to guide vaccine development strategy.Vaccine · 2019Review
- Virulence-Related Genes and Coenteropathogens Associated with Clinical Outcomes of EnteropathogenicJournal of clinical microbiology · 2019Article
- Development of a TaqMan Array card to target 21 purulent meningitis-related pathogens.BMC infectious diseases · 2019Article
- Evaluating next-generation sequencing for direct clinical diagnostics in diarrhoeal disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- xMAP Technology: Applications in Detection of Pathogens.Frontiers in microbiology · 2017Review
- A diagnostic and epidemiologic investigation of acute febrile illness (AFI) in Kilombero, Tanzania.PloS one · 2017Article
- Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.Diagnostic microbiology and infectious disease · 2015Article
- Visual detection of bacterial pathogens via PNA-based padlock probe assembly and isothermal amplification of DNAzymes.Analytical chemistry · 2014Article
- Pentaplex PCR assay for detection of hemorrhagic bacteria from stool samples.Journal of clinical microbiology · 2014Article
- A multiplex PCR/LDR assay for simultaneous detection and identification of the NIAID category B bacterial food and water-borne pathogens.Diagnostic microbiology and infectious disease · 2014Article
- Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens.Journal of clinical microbiology · 2014Article
- Etiology of diarrhea in Bangladeshi infants in the first year of life analyzed using molecular methods.The Journal of infectious diseases · 2013Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
Abstract
Diarrhea can be caused by a range of pathogens, including several bacteria. Conventional diagnostic methods, such as culture, biochemical tests, and enzyme-linked immunosorbent assay (ELISA), are laborious. We developed a 7-plex PCR-Luminex assay to simultaneously screen for several of the major diarrhea-causing bacteria directly in fecal specimens, including pathogenic Aeromonas, Campylobacter jejuni, Campylobacter coli, Salmonella, Shigella, enteroinvasive Escherichia coli (EIEC), Vibrio, and Yersinia. We included an extrinsic control to verify extraction and amplification. The assay was first validated with reference strains or isolates and exhibited a limit of detection of 10(3) to 10(5) CFU/g of stool for each pathogen as well as quantitative detection up to 10(9) CFU/g. A total of 205 clinical fecal specimens from individuals with diarrhea, previously cultured for enteric pathogens and tested for Campylobacter by ELISA, were evaluated. Using these predicate methods as standards, sensitivities and specificities of the PCR-Luminex assay were 89% and 94% for Aeromonas, 89% and 93% for Campylobacter, 96% and 95% for Salmonella, 94% and 94% for Shigella, 92% and 97% for Vibrio, and 100% and 100% for Yersinia, respectively. All discrepant results were further examined by singleplex real-time PCR assays targeting different gene regions, which revealed 89% (55/62 results) concordance with the PCR-Luminex assay. The fluorescent signals obtained with this approach exhibited a statistically significant correlation with the cycle threshold (C(T)) values from the cognate real-time PCR assays (P < 0.05). This multiplex PCR-Luminex assay enables sensitive, specific, and quantitative detection of the major bacterial causes of gastroenteritis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.