ArticleJournal of clinical microbiology2012
Highly specific protease-based approach for detection of porphyromonas gingivalis in diagnosis of periodontitis.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Recent Advances in Microenvironment-Responsive Materials for Periodontitis Therapy.International journal of molecular sciences · 2026Review
- Biosensors in dental, oral and craniofacial applications.Npj biosensing · 2026Review
- Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges.International journal of nanomedicine · 2026Review
- Immunomodulatory effects of eubiotic and dysbiotic multi-species biofilms on oral keratinocytes.BMC oral health · 2025Article
- Identification of Bacterial Networks and Relationship to Host Responses in Early Periodontitis Population over 24 Months.International journal of molecular sciences · 2025Article
- Proteolytic Activity Inhibition in Gingival Fluid by Cysteine Protease Inhibitors Obtained from Egg White andBiomedicines · 2025Article
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- Activatable Photoacoustic Probe for Imaging Infection: Gold Nanorod Dissociation In Vivo Reports Bacterial Protease Activity.ACS nano · 2025Article
- The impact of biocorrosion and titanium ions release on peri-implantitis.Clinical oral investigations · 2025Review
- Molecularly imprinted nanogels as synthetic recognition materials for the ultrasensitive detection of periodontal disease biomarkers.Analytical and bioanalytical chemistry · 2024Article
- Article
- Recent advances in nanomaterial-based biosensor for periodontitis detection.Journal of biological engineering · 2024Review
- Aptamer Based SPREETA Sensor for the Detection ofJournal of microbiology and biotechnology · 2024Article
- Integrating enzyme-nanoparticles bring new prospects for the diagnosis and treatment of immune dysregulation in periodontitis.Frontiers in cellular and infection microbiology · 2024Review
- The effect of sex steroid hormones on the ecology ofBiofilm · 2023Article
- Isolation, characterization, and fibroblast uptake of bacterial extracellular vesicles from Porphyromonas gingivalis strains.MicrobiologyOpen · 2023Article
- Article
- Age-Related Subgingival Colonization ofMicroorganisms · 2023Article
- Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing.ACS applied materials & interfaces · 2023Article
- Precision periodontal care: from omics discoveries to chairside diagnostics.Clinical oral investigations · 2023Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific substrates. Due to the presence of lysine and arginine residues in these substrates, it was hypothesized that the cleavage was mediated by the gingipains, a group of P. gingivalis-specific proteases. This hypothesis was confirmed by the observation that P. gingivalis gingipain knockout strains demonstrated clearly impaired substrate cleavage efficacy. Further, proteolytic activity on the substrates was increased by the addition of the gingipain stimulators dithiothreitol and l-cysteine and decreased by the inhibitors leupeptin and N-ethylmaleimide. Screening of saliva and gingival crevicular fluid of periodontitis patients and healthy controls showed the potential of the substrates to diagnose the presence of P. gingivalis proteases. By using paper points, a sensitivity of approximately 10(5) CFU/ml was achieved. P. gingivalis-reactive substrates fully composed of l-amino acids and Bz-l-Arg-NHPhNO(2) showed a relatively low specificity (44 to 85%). However, the five P. gingivalis-specific substrates that each contained a single d-amino acid showed high specificity (96 to 100%). This observation underlines the importance of the presence of d-amino acids in substrates used for the detection of bacterial proteases. We envisage that these substrates may improve the specificity of the current enzyme-based diagnosis of periodontitis associated with P. gingivalis.
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