ArticleThe Journal of biological chemistry2012
Identification and structural characterization of novel cyclotide with activity against an insect pest of sugar cane.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 98 citations in OpenAlex.
- Molecular insights into anti-cancer cyclotides and their interaction with breast cancer targets: an in silico study.Molecular diversity · 2026Article
- Advances in cyclotide research: bioactivity to cyclotide-based therapeutics.Molecular diversity · 2025Review
- Effects of cyclotide-producing plants on the development and life history parameters of Leipothrix violarius.Experimental & applied acarology · 2025Article
- Decoding small peptides: Regulators of plant growth and stress resilience.Journal of integrative plant biology · 2025Review
- Review
- Small and Versatile Cyclotides as Anti-infective Agents.ACS infectious diseases · 2025Review
- Antimicrobial Plant Peptides: Structure, Classification, Mechanism and Therapeutic Potential.Current topics in medicinal chemistry · 2025Review
- Unveiling the insecticidal efficiency ofHeliyon · 2024Article
- Characterization of cyclotides Mra30 and cycloviolacin O17 derived from Viola dalatensis Gadnep.Archives of microbiology · 2024Article
- Plant peptides - redefining an area of ribosomally synthesized and post-translationally modified peptides.Natural product reports · 2024Review
- Resistance is futile: targeting multidrug-resistant bacteria withRSC chemical biology · 2023Review
- Diversity of transgenes in sustainable management of insect pests.Transgenic research · 2023Review
- The acyclotide ribe 31 from Rinorea bengalensis has selective cytotoxicity and potent insecticidal properties in Drosophila.The Journal of biological chemistry · 2022Article
- Using the Cyclotide Scaffold for Targeting Biomolecular Interactions in Drug Development.Molecules (Basel, Switzerland) · 2022Review
- Plant-Derived Antimicrobial Peptides: Novel Preservatives for the Food Industry.Foods (Basel, Switzerland) · 2022Review
- Nanoparticles in association with antimicrobial peptides (NanoAMPs) as a promising combination for agriculture development.Frontiers in molecular biosciences · 2022Review
- Cyclotide-rich fractions containing nanofibers by electrospinning: preparation, characterization and examination of antimicrobial activity.Turkish journal of chemistry · 2022Article
- Cyclotides from BrazilianJournal of natural products · 2021Article
- Natural Product Inspired Environmentally Friendly Strategy Based on Dopamine Chemistry toward Sustainable Marine Antifouling.ACS omega · 2020Article
- An effective datasets describing antimicrobial peptide produced fromData in brief · 2020Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclotides are a family of plant-derived cyclic peptides comprising six conserved cysteine residues connected by three intermolecular disulfide bonds that form a knotted structure known as a cyclic cystine knot (CCK). This structural motif is responsible for the pronounced stability of cyclotides against chemical, thermal, or proteolytic degradation and has sparked growing interest in this family of peptides. Here, we isolated and characterized a novel cyclotide from Palicourea rigida (Rubiaceae), which was named parigidin-br1. The sequence indicated that this peptide is a member of the bracelet subfamily of cyclotides. Parigidin-br1 showed potent insecticidal activity against neonate larvae of Lepidoptera (Diatraea saccharalis), causing 60% mortality at a concentration of 1 μm but had no detectable antibacterial effects. A decrease in the in vitro viability of the insect cell line from Spodoptera frugiperda (SF-9) was observed in the presence of parigidin-br1, consistent with in vivo insecticidal activity. Transmission electron microscopy and fluorescence microscopy of SF-9 cells after incubation with parigidin-br1 or parigidin-br1-fluorescein isothiocyanate, respectively, revealed extensive cell lysis and swelling of cells, consistent with an insecticidal mechanism involving membrane disruption. This hypothesis was supported by in silico analyses, which suggested that parigidin-br1 is able to complex with cell lipids. Overall, the results suggest promise for the development of parigidin-br1 as a novel biopesticide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.