ArticleProceedings of the National Academy of Sciences of the United States of America2011
Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed.
- Article
- Three-dimensional random-access confocal microscopy with 3D remote focusing system.Communications engineering · 2024Article
- Multifocal fluorescence video-rate imaging of centimetre-wide arbitrarily shaped brain surfaces at micrometric resolution.Nature biomedical engineering · 2024Article
- Spatial and temporal aspects of neuronal calcium and sodium signals measured with low-affinity fluorescent indicators.Pflugers Archiv : European journal of physiology · 2024Review
- Aberration-free holographic microscope for simultaneous imaging and stimulation of neuronal populations.Optics express · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Three-photon excited fluorescence imaging in neuroscience: From principles to applications.Frontiers in neuroscience · 2023Review
- Sensitivity of remote focusing microscopes to magnification mismatch.Journal of microscopy · 2022Article
- Computational optics for high-throughput imaging of neural activity.Neurophotonics · 2022Review
- All-optical interrogation of neural circuits in behaving mice.Nature protocols · 2022Review
- Review
- High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.Neuron · 2022Article
- Comprehensive deep learning model for 3D color holography.Scientific reports · 2022Article
- Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Probing neural codes with two-photon holographic optogenetics.Nature neuroscience · 2021Review
- Precompensation of 3D field distortions in remote focus two-photon microscopy.Biomedical optics express · 2021Article
- Single-objective high-resolution confocal light sheet fluorescence microscopy for standard biological sample geometries.Biomedical optics express · 2021Article
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Access to three-dimensional structures in the brain is fundamental to probe signal processing at multiple levels, from integration of synaptic inputs to network activity mapping. Here, we present an optical method for independent three-dimensional photoactivation and imaging by combination of digital holography with remote-focusing. We experimentally demonstrate compensation of spherical aberration for out-of-focus imaging in a range of at least 300 μm, as well as scanless imaging along oblique planes. We apply this method to perform functional imaging along tilted dendrites of hippocampal pyramidal neurons in brain slices, after photostimulation by multiple spots glutamate uncaging. By bringing extended portions of tilted dendrites simultaneously in-focus, we monitor the spatial extent of dendritic calcium signals, showing a shift from a widespread to a spatially confined response upon blockage of voltage-gated Na(+) channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.