ArticleJournal of neuroinflammation2011
Microglia use multiple mechanisms to mediate interactions with vitronectin; non-essential roles for the highly-expressed αvβ3 and αvβ5 integrins.
Article in Journal of neuroinflammation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular Basis of Glia-ECM Interplay in Central Nervous System Homeostasis and Plasticity.Cells · 2026Review
- TREM2 deficiency causes region-specific brain effects in a mouse model of cerebral amyloid angiopathy.bioRxiv : the preprint server for biology · 2026Article
- FNDC5/irisin ameliorates diabetes-associated cognitive dysfunction via αVβ5-cAMP signaling to regulate oxidative stress, neuroinflammation and synaptic plasticity.Journal of translational medicine · 2026Article
- Reactive astrocyte-derived neurotoxicity is mitigated by vitronectin in traumatic brain injury mouse model.IBRO neuroscience reports · 2025Article
- Critical Role of the Endothelial αV/β5 Integrin Receptor Signaling Pathway in Promoting Irisin-induced Hippocampal BDNF Expression.Molecular neurobiology · 2025Article
- Physical confinement and phagocytic uptake induce persistent cell migration.Biology open · 2025Article
- Article
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- Exercise mimetics: a novel strategy to combat neuroinflammation and Alzheimer's disease.Journal of neuroinflammation · 2024Review
- The role of exercise-related FNDC5/irisin in depression.Frontiers in pharmacology · 2024Review
- TREM2 deficiency in microglia accelerates photoreceptor cell death and immune cell infiltration following retinal detachment.Cell death & disease · 2023Article
- Laminins in tumor-derived exosomes upregulated by ETS1 reprogram omental macrophages to promote omental metastasis of ovarian cancer.Cell death & disease · 2022Article
- Irisin ameliorates neuroinflammation and neuronal apoptosis through integrin αVβ5/AMPK signaling pathway after intracerebral hemorrhage in mice.Journal of neuroinflammation · 2022Article
- IntegrinInternational journal of molecular sciences · 2021Article
- TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.Alzheimer's research & therapy · 2020Article
- Blood vitronectin is a major activator of LIF and IL-6 in the brain through integrin-FAK and uPAR signaling.Journal of cell science · 2018Article
- A microglial hypothesis of globoid cell leukodystrophy pathology.Journal of neuroscience research · 2016Review
- TIMP-1 couples RhoK activation to IL-1β-induced astrocyte responses.Neuroscience letters · 2015Article
- Platelets and platelet alloantigens: Lessons from human patients and animal models of fetal and neonatal alloimmune thrombocytopenia.Genes & diseases · 2015Article
- c-FOS suppresses ovarian cancer progression by changing adhesion.British journal of cancer · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs the primary resident immune cells, microglia play a central role in regulating inflammatory processes in the CNS. The extracellular matrix (ECM) protein vitronectin promotes microglial activation, switching microglia into an activated phenotype. We have shown previously that microglia express two vitronectin receptors, αvβ3 and αvβ5 integrins. As these integrins have well-defined roles in activation and phagocytic processes in other cell types, the purpose of the current study was to investigate the contribution of these two integrins in microglial activation.
methodsMicroglial cells were prepared from wild-type, β3 integrin knockout (KO), β5 integrin KO or β3/β5 integrin DKO mice, and their interactions and activation responses to vitronectin examined in a battery of assays, including adhesion, expression of activation markers, MMP-9 expression, and phagocytosis. Expression of other αv integrins was examined by flow cytometry and immunoprecipitation.
resultsSurprisingly, when cultured on vitronectin, microglia from the different knockout strains showed no obvious defects in adhesion, activation marker expression, MMP-9 induction, or phagocytosis of vitronectin-coated beads. To investigate the reason for this lack of effect, we examined the expression of other αv integrins. Flow cytometry showed that β3/β5 integrin DKO microglia expressed residual αv integrin at the cell surface, and immunoprecipitation confirmed this finding by revealing the presence of low levels of the αvβ1 and αvβ8 integrins. β1 integrin blockade had no impact on adhesion of β3/β5 integrin DKO microglia to vitronectin, suggesting that in addition to αvβ1, αvβ3, and αvβ5, αvβ8 also serves as a functional vitronectin receptor on microglia.
conclusionsTaken together, this demonstrates that the αvβ3 and αvβ5 integrins are not essential for mediating microglial activation responses to vitronectin, but that microglia use multiple redundant receptors to mediate interactions with this ECM protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.